文章摘要
李明科,周景昕,吴延虎.瘦素对主动脉瓣膜间质细胞表型转化的影响[J].南京医科大学学报,2017,(8):976~979
瘦素对主动脉瓣膜间质细胞表型转化的影响
Effects of leptin on aortic valve interstitial cells
投稿时间:2017-01-23  
DOI:10.7655/NYDXBNS20170811
中文关键词: 瘦素  主动脉瓣膜间质细胞  骨桥蛋白  肌动蛋白  碱性磷酸酶
英文关键词: leptin  aortic valvular interstitial cells  OPN  α-SMA  ALP
基金项目:江苏省六大人才高峰(2012-WSW-108)
作者单位
李明科 南京医科大学第一附属医院心脏大血管外科江苏 南京 210029 
周景昕 南京医科大学第一附属医院心脏大血管外科江苏 南京 210029 
吴延虎 南京医科大学第一附属医院心脏大血管外科江苏 南京 210029 
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中文摘要:
      目的:探讨瘦素对主动脉瓣膜间质细胞(valvular interstitial cells,VICs)表型转化的影响,揭示瘦素在钙化性主动脉瓣疾病(calcific aortic value disease,CAVD)发生发展中所扮演的角色。方法:胶原酶消化法分离并培养猪主动脉瓣膜间质细胞,采用含不同浓度的瘦素分别刺激细胞24 h,检测细胞裂解液碱性磷酸酶(ALP)活性,并确定最佳刺激浓度。用最佳刺激浓度的瘦素刺激细胞24 h,提取细胞RNA以及蛋白,通过RT-PCR以及Western blot分别测定肌动蛋白(α-SMA)、骨桥蛋白(OPN)的表达情况。结果:瘦素刺激导致ALP表达上调且最大刺激浓度为100 ng/mL。相较于对照组,瘦素刺激组 OPN mRNA及蛋白表达增高,而α-SMA表达无明显变化。结论:瘦素能够诱导VICs向成骨细胞分化,从而促进VICs钙化。
英文摘要:
      Objective :To investigate the effects of leptin on aortic valvular interstitial cells (VICs) transdifferentiation and reveal the role of leptin in the development of calcific aortic valve disease (CAVD). Method Porcine aortic VICs were separated and cultured via collagenase digestion. The VICs were incubated with leptin in different concentrations for 24 hours and the best stimulation concentration was determined by detecting ALP activity in the cell lysate. After incubated into optimal stimulation concentration for 24 hours, mRNA and protein expressions of α-SMA and OPN were detected by RT-PCR and Western blot, respectively. Results: Treatment with leptin enhanced the ALP expression of VICs and the optimal concentration was 100 ng/ml. Leptin treatment significantly promoted the mRNA and protein expression of OPN while α-SMA was not changed. Conclusion: Leptin can promote VICs calcification through inducing VICs transdifferent into osteoblast-like cells.
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