文章摘要
吉 冰,孙 晔,张 川,王 森,张 悦,孙跃明.ADAM12基因在结直肠癌中的表达及临床意义[J].南京医科大学学报,2017,(8):1015~1018
ADAM12基因在结直肠癌中的表达及临床意义
Clinical significance of expression of ADAM12 gene in patients with colorectal cancer
投稿时间:2016-12-29  
DOI:10.7655/NYDXBNS20170819
中文关键词: ADAM12  结直肠癌  预后
英文关键词: ADAM12  colorectal cancer  prognosis
基金项目:教育部项目(2012YQ030261);江苏省自然科学基金(BK20131448)
作者单位
吉 冰 南京医科大学第一附属医院结直肠外科江苏 南京 210029 
孙 晔 南京医科大学第一附属医院结直肠外科江苏 南京 210029 
张 川 南京医科大学第一附属医院结直肠外科江苏 南京 210029 
王 森 南京医科大学第一附属医院结直肠外科江苏 南京 210029 
张 悦 南京医科大学第一附属医院结直肠外科江苏 南京 210029 
孙跃明 南京医科大学第一附属医院结直肠外科江苏 南京 210029 
摘要点击次数: 437
全文下载次数: 504
中文摘要:
      目的: 研究ADAM12基因在结直肠癌中的表达水平及其与临床参数和预后的关系。方法: 收集50例手术切除的结直肠癌及相应的癌旁组织标本,采用实时荧光定量PCR技术检测标本中ADAM12基因的表达水平,并分析ADAM12基因表达与结直肠癌临床病理特征及预后的关系。同时运用Western blot检测其中5对结直肠癌组织及配对的癌旁组织中ADAM12蛋白的表达情况。结果: ADAM12基因在结直肠癌中mRNA和蛋白水平的表达都显著高于相应的癌旁组织。ADAM12基因在 76.0 %(38/50)的病例中表现为高表达,且其高表达与结直肠癌的淋巴结转移(P=0.005)、TNM分期 (P=0.002)密切相关。高表达ADAM12基因与结直肠癌的3年生存率显著相关。结论:ADAM12基因的表达在结直肠癌中明显上调,且其表达上调与结直肠癌的转移潜能及进展相关,提示ADAM12是结直肠癌的一个潜在分子指标物。ADAM12的高表达与患者的3年生存率显著相关,可作为判断预后的一个重要指标。
英文摘要:
      Objective: To explore the expression pattern of ADAM12 gene in patients with colorectal cancer and analyze its correlation with the clinicopathological parameters and the tumor markers. Methods: ADAM12 expression in mRNA level was detected using quantitative real-time PCR in the colorectal cancer tissues and corresponding adjacent normal tissues from 50 patients. The correlations of ADAM12 expression profile with the clinicopathological parameters and prognosis were evaluated. The expression pattern of ADAM12 gene in protein level was detected using Western blot in the colorectal cancer tissues and corresponding adjacent normal tissues from 5 patients. Results: ADAM12 expression level was significantly higher in the colorectal cancer tissues than in the corresponding adjacent normal tissues,in both protein and mRNA level. Of the 50 patients, 38 (76%) showed up-regulated ADAM12 expression, which correlated significantly with lymphatic metastasis (P=0.005), TNM stage(P=0.002) and survival time(P<0.05) .During the 3-year follow up, 57.89%(22/38)patients in up-regulated died while in normal group,only 17.6%(2/12)died for colorectal cancer(P =0.013). Conclusion: Up-regulation of ADAM12 in colorectal cancer is correlated with lymphatic metastasis and progression and prognosis, suggesting the value of ADAM12 as a potential marker for colorectal cancer.
查看全文   查看/发表评论  下载PDF阅读器
关闭