Deletion of PDK1 impaires the hippocampus-dependent learning and memory in mice
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摘要:
目的:探索3-磷酸肌醇依赖的蛋白激酶-1（3-phosphoinositide-dependent protein kinase-1，PDK1）在海马依赖的学习记忆中的作用。方法:采用Cre/Loxp系统条件性敲除了表达在背侧端脑中Emx1（empty spiracles homeobox 1）阳性神经细胞中的PDK1，利用聚合酶链式反应及行为学实验Morris水迷宫对PDK1敲除小鼠进行研究。结果:该系统确实能敲除海马中的PDK1，且敲除后的小鼠学习记忆能力明显下降。结论: PDK1敲除后影响了小鼠的学习记忆能力，PDK1在海马介导的学习记忆过程中有重要作用。
Abstract:
Objective: To investigate the function of 3-phosphoinositide-dependent protein kinase-1(PDK1) in the hippocampus dependent learning and memory. Methods: We used the Cre-Loxp to knock out PDK1 in empty spiracles homeobox 1(Emx1) positive cells conditionally, which is expressed in the dorsal telencephalon. PCR was used for genotyping and Morris maze was used to test the learning and memory of mice. Results: We found that the PDK1 knockout mice had weaker capacity of learning and memory associated with hippocampus. Conclusion: PDK1 plays an important role in the hippocampus-dependent learning and memory in mice.
