Objective：To investigate the value of interleukin 18 (IL-18) and urinary kidney injury molecule 1 (KIM-1) in blood and urine in early diagnosis of acute kidney injury (AKI) after coronary intervention. Methods: A total of 243 eligible patients who underwent coronary intervention were divided into the AKI group (48 cases) and the non-AKI group (195 cases) according to the diagnostic criteria of AKI. Blood and urine samples were collected at different time points (0, 2, 4, 6, 8, 10, 12, 24, 48, and 72 h) after coronary intervention, and then the concentrations of serum creatinine(SCr), IL-18 and KIM-1 in serum and urine were measured. Results: The levels of SCr from 12 h to 72 h were higher than that of 0 h in the AKI group(all P<0.05). The levels of IL-18 and KIM in serum and urine were significantly higher than those of 0 h. IL-18 level reached peak at 2 h(blood) or 4 h(urine), and KIM-1 level reached peak at 6 h (blood) or 8 h (urine) (all P<0.05). Pearson correlation analysis showed the levels of 2 h-serum IL-18, 4 h-urine IL-18, 6 h-serum KIM-1 and 8 h-urine KIM-1 were positively correlated with that of SCr(24 h) (all P<0.05). The ROC analysis showed the levels of IL-18 and KIM-1 in serum and urine had a good diagnostic efficiency. The joint detection had a higher AUC than single marker detection. Conclusion： The joint detection of 2 h-serum IL-18, 4 h-urine IL-18, 6 h-serum KIM-1 and 8 h-urine KIM-1 after intervention had a better diagnostic performance than single marker detection, which may be an important reference for early diagnosis of AKI.