文章摘要
宋 虎,张亚飞,徐 为,徐溢新,宋 军.FBXO22对结肠癌细胞侵袭迁移的影响及相关机制[J].南京医科大学学报,2017,(12):1553~1556
FBXO22对结肠癌细胞侵袭迁移的影响及相关机制
Effect of silencing FBXO22 gene on invasion and migration and related molecular mechanism in colon cancer cells
投稿时间:2017-05-13  
DOI:10.7655/NYDXBNS20171203
中文关键词: FBXO22基因  结肠癌  侵袭  迁移
英文关键词: FBXO22  colon cancer  invasion  migration
基金项目:江苏省“六大人才高峰”项目(2014-WSW-032);江苏省“科教强卫工程”青年医学人才项目资助(QNRC2016790)
作者单位
宋 虎 徐州医科大学附属医院胃肠外科江苏 徐州 221002 
张亚飞 徐州医科大学附属医院胃肠外科江苏 徐州 221002 
徐 为 徐州医科大学附属医院胃肠外科江苏 徐州 221002 
徐溢新 徐州医科大学附属医院胃肠外科江苏 徐州 221002 
宋 军 徐州医科大学附属医院胃肠外科江苏 徐州 221002 
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中文摘要:
      目的: 探讨结肠癌细胞FBXO22基因表达沉默后对细胞侵袭迁移的影响及其相关分子机制。方法:利用siRNA-Ctrl和siRNA-FBXO22小干扰片段转染结肠癌SW620和HCT116细胞,干扰FBXO22基因表达,Western blot 检测细胞转染效率,Transwell细胞迁移实验分析干扰FBXO22对细胞侵袭迁移的影响,Western blot检测细胞侵袭相关蛋白MMP-2、MMP-9和MDM2水平的变化。结果:转染FBXO22 siRNA后,结肠癌SW620和HCT116细胞FBXO22的表达量明显下降、细胞侵袭转移能力降低,MMP-2和MMP-9蛋白表达水平明显降低,而MDM2蛋白表达水平上调。结论: FBXO22与结肠癌细胞侵袭迁移相关,其机制可能通过调节MMP-2和MMP-9表达而实现。
英文摘要:
      Objective: To explore the effects of FBXO22 silencing by small interfering RNA (siRNA) on invasion and migration of colon cancer cells, and futher to investigate its related molecular mechanism. Methods: The siRNA-Ctrl and siRNA-FBXO22 fragments were transfected into SW620 and HCT116 cells, and the level of FBXO22 was detected by Western bolt. The effects of interfering FBXO22 on cell invasion and migration were analyzed by Transwell assay, and the expression levels of FBXO22, MMP-2, MMP-9 and MDM2 were detected by Western bolt. Results: After transfection with FBXO22 siRNA, the levels of FBXO22 in SW620 and HCT116 cells were significantly decreased, and the abilities of cell invasion and migration were decreased. Meanwhile, silencing of FBXO22 decreased expression of MMP-2, MMP-9, and increased expression of MDM2. Conclusion: FBXO22 can affect cell invasion and migration in colon cancer cells, and the mechanism may be related to the expression of MMP-2 and MMP-9.
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