文章摘要
方 川,黄 凯,檀艳丽,康春生,张建宁.人脑胶质母细胞瘤PDX模型的建立[J].南京医科大学学报,2018,(1):11~13
人脑胶质母细胞瘤PDX模型的建立
The establishment of patient derived xenograft of human glioblastoma multiforme
投稿时间:2017-10-20  
DOI:10.7655/NYDXBNS20180103
中文关键词: 胶质母细胞瘤  PDX  个体化治疗  临床前研究
英文关键词: glioblastoma multiforme  PDX  personalized treatment  preclinical research
基金项目:国家重点研究和发展项目(2016YFC0902502);国家自然科学基金(81772667);天津市科委(15ZXJZSY00040);河北省临床医学人才和基础研究项目培训(361007)
作者单位
方 川 天津市神经病学研究所教育部中枢神经系统创伤修复与再生重点实验室天津 300052 
黄 凯 天津市神经病学研究所教育部中枢神经系统创伤修复与再生重点实验室天津 300052 
檀艳丽 河北大学基础医学院河北 保定 071000 
康春生 天津市神经病学研究所教育部中枢神经系统创伤修复与再生重点实验室天津 300052 
张建宁 天津市神经病学研究所教育部中枢神经系统创伤修复与再生重点实验室天津 300052 
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中文摘要:
      目的:探讨患者来源的异种移植(patient derived xenograft,PDX)胶质母细胞瘤模型的建立方法,为临床和生物学研究提供重要的理论依据和技术支持。方法:收集胶质母细胞瘤组织,建立皮下肿瘤模型,待皮下肿瘤生长取出肿瘤,分散肿瘤细胞并种于小鼠颅内。HE染色比较原发肿瘤组织与PDX肿瘤形态学特点,免疫组织化学染色方法检测Ki?67的表达,比较肿瘤增殖能力。结果:成功建立人脑胶质母细胞PDX模型,原发肿瘤和PDX肿瘤形态学特点相似,PDX肿瘤保留了原发肿瘤的增殖能力。结论:通过建立PDX模型,可以将PDX作为胶质母细胞瘤患者个体化治疗的重要临床前研究工具。
英文摘要:
      Objective:To establish a method of patient derived xenograft(PDX)of human glioblastoma multiforme(GBM)for preclinical and biological research. Methods:GBM samples were freshly collected and planted subcutaneously. After the establishment of subcutaneous xenograft,tumor was taken out,dispersed and injected to the mice brain. HE staining was used to compare the morphological characteristics between primary tumor and PDX tumor,and Ki?67 was detected by immunohistochemistry to compare their proliferation activity. Results:We successfully established the PDX model,and found that the morphological characteristics of PDX by HE staining were similar with primary tumor,PDX remained the proliferation activity of primary tumor. Conclusion:The establishment of PDX model enabled us as preclinical tool for personalized treatment of GBM patients.
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