文章摘要
刘伊扬,钱 健,徐爱民,苏仕峰,秦 超,王增军.长链非编码RNA BX357664对肾透明细胞癌细胞上皮间质转化过程的影响[J].南京医科大学学报,2018,(1):25~30
长链非编码RNA BX357664对肾透明细胞癌细胞上皮间质转化过程的影响
LncRNA BX357664 inhibits epithelial⁃to⁃mesenchymal transition in renal cell carcinoma cells
投稿时间:2015-10-19  
DOI:10.7655/NYDXBNS20180106
中文关键词: 长链非编码RNA  上皮间质转化  肾癌
英文关键词: lncRNA  epithelial⁃to⁃mesenchymal transition  renal cell carcinoma
基金项目:国家自然科学基金(81372757)
作者单位
刘伊扬 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
钱 健 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
徐爱民 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
苏仕峰 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
秦 超 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
王增军 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
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中文摘要:
      目的:探讨长链非编码RNA(long noncoding RNA,lncRNA)BX357664在肾癌组织中的表达并研究其对Caki?1和Caki?2细胞上皮间质转化过程的影响。方法:以前期芯片检测结果经标准化分析确认在肾癌组织中表达明显低于癌旁正常组织的lncRNA BX357664为目标,采用逆转录-聚合酶链反应(qRT?PCR)检测其在24对肾癌组织和Caki?1以及Caki?2细胞中的表达情况;lncRNA BX357664慢病毒载体感染Caki?1和Caki?2细胞后,Transwell迁移和侵袭实验分别检测细胞迁移和侵袭能力的改变;Western blot检测Caki?1和Caki?2细胞处理组和对照组中上皮间质转化相关蛋白的表达情况。结果:lncRNA BX357664在24对肾癌组织和Caki?1、Caki?2细胞中表达水平降低(P < 0.05);lncRNA BX357664慢病毒过表达载体感染Caki?1和Caki?2细胞后能够显著抑制细胞迁移(P < 0.01)和侵袭能力(P < 0.01),显著改变上皮间质转化相关蛋白的表达,抑制上皮间质转化的进程(P < 0.05)。结论:lncRNA BX357664在肾癌中显著低表达,在Caki?1和Caki?2细胞中上调lncRNA BX357664能够通过抑制上皮间质转化过程,从而抑制肿瘤细胞的转移和侵袭。
英文摘要:
      Objective:To investigate the expression of lncRNA BX357664 in human renal cell carcinoma (RCC) and its function in epithelial?to?mesenchymal transition (EMT) in Caki?1 and Caki?2 RCC cells. Methods: Downregulated LncRNA BX357664 was confirmed in RCC tumor tissues by previous lncRNA microarray. Real?time PCR was performed to analyze the expression of lncRNA BX357664 in 24 pairs of human RCC samples, Caki?1 and Caki?2 cells, respectively. Transwell migration and invasion assays were performed to detect the ability of migration and invasion in transfected Caki?1 and Caki?2 cells, respectively. Western blotting assay was carried out to identify the relative proteins in EMT process which affected RCC metastasis by lncRNA BX357664. Results: LncRNA BX357664 was downregulated in 24 pairs of RCC samples, Caki?1 and Caki?2 RCC cells, respectively (both P < 0.05). Overexpression of lncRNA BX357664 suppressed migration (P < 0.01) and invasion (P < 0.01) by inhibiting EMT in Caki?1 and Caki?2 cells. The expressions of EMT related proteins were significantly changed, and the process of EMT was inhibited (P < 0.05). Conclusion: LncRNA BX357664 was downregulated in RCC and could inhibit the ability of migration and invasion in Caki?1 and Caki?2 cells by blocking EMT.
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