文章摘要
葛雨秋,金 经,马高祥,邵 卫,储海燕,王美林,张正东.前列腺癌易感位点rs2252004的eQTL和meQTL研究[J].南京医科大学学报,2018,(1):31~33,87
前列腺癌易感位点rs2252004的eQTL和meQTL研究
eQTL and meQTL analysis of prostate cancer susceptibility locus rs2252004
投稿时间:2016-01-27  
DOI:10.7655/NYDXBNS20180107
中文关键词: rs2252004  甲基化  FGFR2  前列腺癌
英文关键词: rs2252004  methylation  FGFR2  prostate cancer
基金项目:国家自然科学基金(81473050),江苏省公共卫生与预防医学优势学科
作者单位
葛雨秋 南京医科大学公共卫生学院职业医学与环境卫生学系江苏 南京 211166 
金 经 南京医科大学公共卫生学院职业医学与环境卫生学系江苏 南京 211166 
马高祥 南京医科大学公共卫生学院职业医学与环境卫生学系江苏 南京 211166 
邵 卫 南京医科大学公共卫生学院职业医学与环境卫生学系江苏 南京 211166 
储海燕 南京医科大学公共卫生学院职业医学与环境卫生学系江苏 南京 211166 
王美林 南京医科大学公共卫生学院职业医学与环境卫生学系江苏 南京 211166 
张正东 南京医科大学公共卫生学院职业医学与环境卫生学系江苏 南京 211166 
摘要点击次数: 402
全文下载次数: 862
中文摘要:
      目的:全基因组关联性研究(GWAS)发现rs2252004与前列腺癌的易感性相关,本研究拟进一步探讨rs2252004参与前列腺癌发生的可能分子生物学机制。方法:运用TCGA公共数据库中的基因型、甲基化、基因表达数据,采用线性回归分析rs2252004基因型与其上下游1 Mb区域CpG位点的甲基化水平及相关基因表达的关联性。结果:rs2252004与FGFR2基因上的3个CpG位点的甲基化水平显著相关(cg25294906,P=0.031;cg03552039,P=0.042;cg16499947,P=0.049)。而FGFR2在50例配对的前列腺癌组织和癌旁组织中表达差异有统计学意义(P=9.3×10-11)。与GG基因型相比,rs2252004 GT基因型的前列腺癌患者的FGFR2基因表达量显著降低(P=0.007)。此外,GT/TT基因型与GG基因型患者的FGFR2表达量差异有统计学意义(P=0.006)。结论:rs2252004可能通过影响FGFR2甲基化水平,进而调控FGFR2基因的表达,从而参与前列腺癌的发生。
英文摘要:
      Objective:Previous genome ? wide association study(GWAS)has identified that rs2252004 was associated with the susceptibility of prostate cancer. This study was aimed to investigate the molecular biological mechanisms underlying the effect of rs2252004 on tumorigenesis of prostate cancer. Methods:We obtained the genotype,methylation and gene expression data from The Cancer Genome Atlas(TCGA)database. The relationships between rs2252004 genotype(0,1,or 2)and the β?value or proportion of methylation at each CpG site(within 500 kb of rs2252004)and the expression level of involved genes were evaluated via linear regression. Results:The results showed that rs2252004 was significantly associated with the methylation level of three CpG cites located in FGFR2(cg25294906,P=0.031;cg03552039,P=0.042;cg16499947,P=0.049,respectively). Furthermore,the expression of FGFR2 remarkably differed between 50 paired prostate tumors and adjacent normal tissues(P=9.3×10-11). Besides,individuals carrying rs2252004 GT genotypes were significantly associated with decreased expression of FGFR2 than those with GG genotypes(P=0.007). We also observed a notable different expression of FGFR2 between GT/TT genotype and GG genotype individuals(P=0.006). Conclusion:Altogether,our results suggest that rs2252004 may regulate the methylation level of FGFR2,which contributes to influence FGFR2 expression,thus be involved in the tumorigenesis of prostate cancer.
查看全文   查看/发表评论  下载PDF阅读器
关闭