Objective：To investigate the prognostic value of microRNA-107（miRNA-107）for patients with penile-sparing surgery.Methods：The clinical data of 34 patients with penile-sparing surgery were collected from June，2009 to June，2014 in the first affiliated hospital of Nanjing Medical University. Kaplan - Meier method was used to analyze the survival data. Univariable analyses and multivariable analyses by Cox regression were performed to analyze the correlation between the potential factors and the prognosis.The factors included age，marital status，history of smoking，histological grade，TNM stage，tumor position，and miRNA - 107 expression.Results：We found that miRNA - 107 expression was associated with histological grade（χ2=3.948，P=0.047）. Univariable analyses showed that higher histological grade（hazard ratio，HR=5.537，P=0.007）and elevated expression of miRNA-107（HR=7.651，P=0.001）significantly correlated with worse prognosis. Multivariable analyses demonstrated that higher miRNA - 107 expression was related to unfavorable prognosis for patients（HR=10.391，P=0.003）. Conclusion：miRNA-107 is an independent prognostic factor for patients with penile-sparing surgery，and it could be considered as an important biomarker.