文章摘要
夏 阳,陈慧敏,胡姝颖,孙剑飞,郭 宇,马珊珊.联合应用纳米金和磷酸钙骨水泥支架促进牙髓干细胞的增殖和成骨分化[J].南京医科大学学报,2018,(5):590~594
联合应用纳米金和磷酸钙骨水泥支架促进牙髓干细胞的增殖和成骨分化
Calcium phosphate cement scaffold with gold nanoparticleson promotes the proliferation and osteogenic differentiation of dental pulp stem cells
投稿时间:2017-07-07  
DOI:10.7655/NYDXBNS20180504
中文关键词: 纳米金  磷酸钙骨水泥  牙髓干细胞  增殖  成骨分化
英文关键词: gold nanoparticles  calcium phosphate cement  dental pulp stem cells  proliferation  osteogenic differentiation
基金项目:国家自然科学基金(81771044);中国博士后基金(2015M571647);江苏省博士后基金(1402044B);江苏高校优势学科建设工程资助项目(2014?37);江苏省青年医学人才(QNRC2016853)
作者单位
夏 阳 东南大学江苏省生物材料与器件重点实验室江苏 南京 210009南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
陈慧敏 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
胡姝颖 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
孙剑飞 东南大学江苏省生物材料与器件重点实验室江苏 南京 210009 
郭 宇 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
马珊珊 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
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中文摘要:
      目的:磷酸钙骨水泥(calcium phosphate cement,CPC)支架以其良好的生物相容性和骨引导活性而广泛应用于组织工程骨缺损的修复中,但是其骨引导活性有限。因此考虑联合应用纳米金(gold nanoparticles,AuNPs)以促进牙髓干细胞的增殖和成骨分化。方法:以CPC支架作为基底接种牙髓干细胞,在培养基中添加5 μg/mL AuNPs进行干细胞培养,以常规培养基组为对照。用透射电镜检测AuNPs的形貌。以荧光显微镜检测细胞接种4 h后的黏附状态,以CCK?8检测细胞增殖情况,检测细胞碱性磷酸酶的活性,并进行茜素红染色以说明矿化物形成情况。结果:AuNPs为均匀的球形,直径20 nm左右。细胞接种后4 h,实验组和对照组的细胞黏附状态没有显著差异。CPC上AuNPs培养基中的细胞14 d时数量多于对照组(P < 0.05);且AuNPs培养基中细胞的碱性磷酸酶活性显著增高(P < 0.05),矿化物的形成也显著增多(P < 0.05)。结论:CPC支架联合AuNPs可显著促进牙髓干细胞的增殖和成骨分化。
英文摘要:
      Objective:Calcium phosphate cement(CPC)scaffold is widely used in bone tissue engineering due to its good biocompatibility and osteoconduction properties. However,it needs further improvements to widen its application. In this study,CPC scaffold with gold nanoparticles(AuNPs)were used to promote the proliferation and osteogenic differentiation of dental pulp stem cells(DPSCs). Methods:The morphology of AuNPs was detected by transmission electron microscope(TEM). The cells were seeded on CPC,and cultured in media containing 5 μg/mL AuNPs. Those seeded on CPC and cultured in normal media was set as control. The cell adhesion was detected by fluorescent stain 4 h after seeding. The cell proliferation was detected by CCK?8,and the alkaline phosphatase(ALP)activity was measured. Alizarin red staining(ARS)was used to detect the mineral synthesis. Results:TEM image showed that AuNPs were spherical with 20 nm in average diameter. Fluorescent images showed no significant difference in cell adhesion between the two groups. Significant difference in cell proliferation was detected at 14 days(P < 0.05). ALP activity of the AuNPs media group was significantly increased(P < 0.05),and the mineralization formation of DPSCs was also increased compared with that of cells in normal media(P < 0.05). Conclusion:It was concluded that CPC scaffold with AuNPs can promote the proliferation and osteogenic differentiation of DPSCs.
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