文章摘要
汤井源,蒋旭平,秦志强,韩 鹏,秦 超,沈百欣,王 巍,唐 敏,张 炜.小鼠邻苯二甲酸二丁酯染毒模型建立及莱菔硫烷保护作用的探索[J].南京医科大学学报,2018,(5):595~599
小鼠邻苯二甲酸二丁酯染毒模型建立及莱菔硫烷保护作用的探索
Establishment of dibutyl phthalate poisoning mouse model and the protective response of sulforaphane
投稿时间:2017-11-03  
DOI:10.7655/NYDXBNS20180505
中文关键词: 邻苯二甲酸二丁酯  莱菔硫烷  生殖毒性  动物模型
英文关键词: dibutyl phthalate  sulforaphane  reproductive toxicity  animal model
基金项目:国家自然科学基金(81670608,81600514)
作者单位
汤井源 南京中医药大学附属医院江苏省中医院泌尿外科江苏 南京 210029 
蒋旭平 宜兴市人民医院泌尿外科江苏 宜兴 214200 
秦志强 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
韩 鹏 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
秦 超 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
沈百欣 南京医科大学第二附属医院泌尿外科江苏 南京 210011 
王 巍 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
唐 敏 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
张 炜 南京医科大学第一附属医院泌尿外科江苏 南京 210029 
摘要点击次数: 314
全文下载次数: 266
中文摘要:
      目的:用邻苯二甲酸二丁酯(DBP)建立小鼠染毒模型并探讨莱菔硫烷(sulforaphane,SFN)的保护作用。 方法:将30只6周龄小鼠随机分为溶剂对照组(大豆油组)、SFN组[0.75 mg/(kg·d)]、DBP组[500 mg/(kg·d)]、DBP+SFN低剂量组[0.5 mg/(kg·d)]、DBP+SFN中剂量组[0.75mg/(kg·d)]、DBP+SFN高剂量组[1.00 mg/(kg·d)],每组5只,连续喂药2周后处死,测量睾丸重量、肛殖距(AGD)、AGD/体重、睾丸脏器系数等指标,并观察小鼠睾丸的组织病理学改变。另取一侧附睾中精子进行精子计数、精子活力以及精子畸形率分析。结果:相比于对照组,DBP组AGD/体重、精子计数、活力等指标均明显降低,精子畸形率显著升高(P < 0.05),睾丸出现明显病理改变。而DBP+SFN低剂量组各检测指标均优于DBP组,差异有统计学意义(P < 0.05),SFN能够明显改善DBP 导致的小鼠睾丸组织病变。结论:SFN可显著缓解DBP导致的生殖毒性,为进一步探讨DBP毒性作用和SFN的保护机制提供了合适的动物实验模型。
英文摘要:
      Objective:To establish the dibutyl phthalate(DBP)poisoning mouse model and discuss the protective response of sulforaphane(SFN). Methods:A total of 30 male mice with age of 6 weeks old were randomly divided into the solvent control group(soybean oil group),the SFN group[0.75 mg/(kg·d)],the DBP group[500 mg/(kg·d)],the DBP with SFN low dose group[0.5 mg/(kg·d)],the DBP with SFN middle dose group[0.75 mg/(kg·d)]and the DBP with SFN high dose group[1 mg/(kg·d)]with 5 mice in each. After continuous feeding drugs for 2 weeks,all mice were sacrificed and anatomized,the testis weight,anogenital distance(AGD),AGD/weight and the organ coefficients of testis were measured. In addition,the testicular histopathological changes of mice were observed and one side of epididymis was used to analysis the sperm counts,viability and deformity rate. Results:The DBP group presented with obvious lower AGD/weight,sperm counts,viability and higher deformity rate,significant testicular histopathological changes compared to the soybean oil group(P < 0.05). However,all the test results in the DBP with SFN low dose group were superior to that of the DBP group(P < 0.05)and the testicular histopathological changes were improved significantly. Conclusion:SFN could alleviate the reproductive toxicity induced by DBP significantly and this provides an experimental animal model for further studying the toxicity of DBP and the protective mechanism of SFN.
查看全文   查看/发表评论  下载PDF阅读器
关闭