文章摘要
王玉珍,陈 旭,吴偲偲,葛 舒,徐 艳,叶 宇.Del⁃1在人牙周膜成纤维细胞中的表达及相关炎症机制初探[J].南京医科大学学报,2018,(7):915~921,927
Del⁃1在人牙周膜成纤维细胞中的表达及相关炎症机制初探
Expression of Del⁃1 in human periodontal ligament cells and its related inflammatory mechanism
投稿时间:2018-02-25  
DOI:10.7655/NYDXBNS20180709
中文关键词: 牙周膜成纤维细胞  Del⁃1/Edil 3  炎症  内毒素
英文关键词: periodontal ligament cells  Del⁃1/EDIL3  inflammation  LPS
基金项目:国家自然科学基金(81771074,81470749);江苏省高校自然科学研究重大项目(16KJA320001);江苏省高层次卫生人才“六个一工程”(LGY2016048);江苏省高校优势学科建设工程资助(2014-37)
作者单位
王玉珍 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
陈 旭 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
吴偲偲 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
葛 舒 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
徐 艳 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
叶 宇 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
摘要点击次数: 994
全文下载次数: 1469
中文摘要:
      目的:检测Del?1蛋白在人牙周膜成纤维细胞中的表达情况,并观察内毒素(lipopolysaccharides,LPS)对人牙周膜成纤维细胞Del?1表达的影响,探讨其在牙周炎进展中的可能作用机制。方法:组织块法取人牙周膜成纤维细胞,用细胞免疫荧光、流式细胞术及体外管腔形成试验鉴别细胞来源,细胞免疫荧光检测Del?1蛋白在人牙周膜成纤维细胞中的表达情况;用不同浓度LPS刺激细胞,检测Del?1蛋白的表达水平,以Del?1减少最显著的LPS浓度为最佳刺激浓度,再用该浓度的LPS刺激细胞不同时间,检测Del?1及炎症因子IL?6的表达水平;用不同浓度的Del?1蛋白预刺激细胞1 h,再用最佳浓度的LPS刺激细胞24 h,检测炎症因子IL?6的表达情况;用最适宜浓度的Del?1蛋白预刺激细胞1 h,再用LPS刺激细胞10 min,检测IκBα的激活情况。结果:Del?1蛋白在人牙周膜成纤维细胞中有表达,定位于细胞的胞浆中;LPS刺激细胞时Del?1的表达水平降低,并随浓度的升高和处理时间的延长而下降,IL?6的表达与之相反;Del?1蛋白可下调LPS诱导的炎症因子IL?6的表达,抑制IκBα的磷酸化。结论:本研究首次发现Del?1可以在人牙周膜成纤维细胞中表达,且在LPS刺激人牙周膜成纤维细胞时表达量降低,Del?1蛋白可通过抑制NF?κB通路的激活,在牙周炎的发生发展中发挥拮抗作用。
英文摘要:
      Objective:To investigate whether Del?1 is expressed in human periodontal ligament cells(hPDLCs),and the effect of lipopolysaccharides(LPS)on the expression of Del?1 in hPDLCs,and to explore the potential mechanism of Del?1 in the development of periodontitis. Methods:HPDLCs were isolated and cultured by tissue block method,and immunofluorescence assay,flow cytometry and Matrigel assay were used to identify cells. Immunofluorescence was used to detect if Del?1 was expressed in hPDLCs. Then variable concentrations of LPS were used to stimulate hPDLCs,and the expression of Del?1 was detected. The optimal concentration of LPS was selected,based on the most significant decrease of Del?1. The optimal concentration was selected to be used in the time?dependent experiment. The expressions of Del?1 and IL?6 were respectively assayed. hPDLCs were pretreated with Del?1 of variable concentrations(0,0.05,0.5 and 5 μg/mL)for 1 h,and then stimulated with LPS for 24 h. The expressions of IL?6 were assayed. hPDLCs were pretreated with the optimal concentration of Del?1 for 1 h,and then stimulated with LPS for 10 min,for detecting the phosphorylation of IκBα. Results:Del?1 could be expressed in hPDLCs,locating in cytoplasm of hPDLCs. The expressions of Del?1 were significantly down?regulated by LPS in a dose?independent and time?independent manner,which were contrary to IL?6. Del?1 could down?regulate the expression of IL?6 which was induced by LPS and inhibit the phosphorylation of IκBα in hPDLCs. Conclusions:Our study discovered for the first time that Del?1 could be expressed in hPDLCs,and it was down?regulated by LPS in hPDLCs,Del?1 could suppress the phosphorylation of NF?κB,playing an antagonistic role in the development of periodontitis.
查看全文   查看/发表评论  下载PDF阅读器