文章摘要
王振婷,吴偲偲,吴程宇,燕 珂,徐 艳,陈 旭.TRAF6敲低对低氧状态下IL⁃17调控人牙周膜成纤维细胞表达OPG及RANKL的影响[J].南京医科大学学报,2018,(7):922~927
TRAF6敲低对低氧状态下IL⁃17调控人牙周膜成纤维细胞表达OPG及RANKL的影响
Effects of TRAF6 knockdown on the IL⁃17 modulating the expression of OPG and RANKL in periodontal ligament cells under hypoxia
投稿时间:2018-02-27  
DOI:10.7655/NYDXBNS20180710
中文关键词: 牙周炎  TRAF6  RANKL  OPG  低氧
英文关键词: periodontitis  TRAF6  RANKL  OPG  hypoxia
基金项目:国家自然科学基金(81771074,81470749);江苏省高校自然科学研究重大项目(16KJA320001);江苏省高层次卫生人才“六个一工程”(LGY2016048);江苏省高校优势学科建设工程资助项目(2014?37)
作者单位
王振婷 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
吴偲偲 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210030 
吴程宇 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210031 
燕 珂 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210032 
徐 艳 南京医科大学口腔疾病研究江苏省重点实验室2南京医科大学附属口腔医院牙周科江苏 南京 210029 
陈 旭 南京医科大学口腔疾病研究江苏省重点实验室2南京医科大学附属口腔医院牙周科江苏 南京 210029 
摘要点击次数: 966
全文下载次数: 887
中文摘要:
      目的:探讨敲低肿瘤坏死因子受体相关因子6(tumor necrosis factor receptor?associated factor 6,TRAF6)对人牙周膜成纤维细胞(human periodontal ligament cells,hPDLCs)在低氧状态经白介素17(interleukin 17,IL?17)刺激表达骨保护素(osteoprotegerin,OPG)及核因子κB受体活化因子配体(receptor activator for nuclear factor?κB ligand,RANKL)的影响。方法:将hPDLCs细胞设为常氧加IL?17组、低氧加IL?17组以及单纯低氧组,每组内分为阴性对照组(TRAF6?NC组)和干扰组(TRAF6?shRNA组)。将靶向TRAF6基因的shRNA慢病毒载体(TRAF6?shRNA)感染hPDLCs,通过倒置显微镜观察病毒感染效率并用RT?PCR方法验证,用Western blot及RT?PCR方法检测分析hPDLCs低氧诱导因子(hypoxia inducible factor?1,HIF?1)、OPG及RANKL的表达变化。结果:低氧加IL?17组RANKL与OPG蛋白相对表达量的比值RANKL/OPG高于常氧加IL?17组及低氧组。在低氧加IL?17条件下,与TRAF6?NC组相比,TRAF6?shRNA组RANKL表达减少,RANKL与OPG相对表达量的比值RANKL/OPG减少。结论:TRAF6参与低氧状态下IL?17刺激hPDLCs表达骨吸收分子的调控,敲低TRAF6可使低氧状态下IL?17诱导的hPDLCs表达骨吸收分子减少。
英文摘要:
      Objective:This study aimed at the effect of tumor necrosis factor receptor?associated factor 6(TRAF6)knockdown on interleukin?17(IL?17)modulating the expression of osteoprotegerin(OPG)and receptor activator for nuclear factor?κB ligand(RANKL)in human periodontal ligament cells(hPDLCs)under hypoxia. Methods:The experiment was set as the normoxia plus IL?17 group,the hypoxia plus IL?17 group and the hypoxia group. Each group was divided into the negative control group(the TRAF6?NC group)and the disturbance group(the TRAF6?shRNA group). The shRNA lentiviral vector was used to infect hPDLCs. The efficiency of infection was observed by inverted microscope and verified by RT?PCR. The expression of HIF?1α,RANKL and OPG in human periodontal ligament cells were detected by Western blot and RT?PCR. Results:The ratio of RANKL/OPG relative expression of RANKL and OPG protein in the hypoxia plus IL?17 group was higher than that in the hypoxia group. Under hypoxia plus IL?17 conditions,the expression of RANKL was decreased in the TRAF6?shRNA group compared with the TRAF6?NC group,and the ratio of RANKL/OPG relative expression of RANKL and OPG was decreased. Conclusion:TRAF6 is involved in the regulation of IL?17?stimulated expression of bone resorption molecules in human periodontal ligament cells in hypoxic conditions. Knocking down TRAF6 can reduce IL?17?induced expression of bone resorption molecules in human periodontal ligament cells.
查看全文   查看/发表评论  下载PDF阅读器