文章摘要
吴康健,吴假假,曾 凯,张晓辉,魏 威,许利剑.MicroRNA⁃141靶向致癌基因Bmi⁃1抑制胰腺癌细胞的增殖[J].南京医科大学学报,2018,(7):950~955
MicroRNA⁃141靶向致癌基因Bmi⁃1抑制胰腺癌细胞的增殖
miR⁃141;Bmi⁃1;细胞增殖;胰腺癌
投稿时间:2018-03-08  
DOI:10.7655/NYDXBNS20180715
中文关键词: miR⁃141  Bmi⁃1  细胞增殖  胰腺癌
英文关键词: miR⁃141  Bmi⁃1  cell proliferation  pancreatic cancer
基金项目:江苏省卫计委面上项目(H201409);江苏省“六大人才高峰”资助(WAW?008)
作者单位
吴康健 南京医科大学第二附属医院普外科江苏 南京 210011 
吴假假 南京医科大学第二附属医院普外科江苏 南京 210011 
曾 凯 南京医科大学第二附属医院普外科江苏 南京 210011 
张晓辉 南京医科大学第二附属医院普外科江苏 南京 210011 
魏 威 南京医科大学第二附属医院普外科江苏 南京 210011 
许利剑 南京医科大学第二附属医院普外科江苏 南京 210011 
摘要点击次数: 1032
全文下载次数: 895
中文摘要:
      目的:探讨miR?141调控胰腺癌细胞增殖的机制。方法:采用qRT?PCR检测正常胰腺细胞和4种胰腺癌细胞株中miR?141的表达。从Gene Expression Omnibus数据库(GEO)下载包含36例胰腺癌样本和16例正常样本的GSE71533 miR?141表达文件,分析miR?141在胰腺癌及癌旁的表达差异。Western blot 检测Bmi?1在正常胰腺细胞和4种胰腺癌细胞株中的表达。荧光素酶报告基因检测miR?141和Bm?1之间的靶向关系。通过CCK8、平板克隆形成检测Bmi?1和miR?141对胰腺癌细胞增殖能力的影响。结果:qRT?PCR显示与正常胰腺细胞相比,miR?141在胰腺癌细胞中表达下调;GSE71533数据集分析结果也显示miR?141在胰腺癌组织中下调;Western blot分析结果表明Bmi?1在胰腺癌细胞中高表达,双荧光素酶实验表明miR?141锚定在Bmi?1的3′非编码区,下调Bmi?1可以抑制胰腺癌细胞的增殖;上调miR?141可以降低Bmi?1的蛋白质表达水平,从而抑制胰腺癌细胞中细胞增殖。结论:miR?141通过靶向Bmi?1在胰腺癌中起抑制作用,在胰腺癌诊疗中具有潜在功能。
英文摘要:
      Objective:To investigate the mechanism of miR?141 regulating cell proliferation in pancreatic cancer. Methods:Expression of miR?141 in normal pancreas cell and four pancreatic cancer cell lines were detected by quantitative reverse transcription PCR(qRT?PCR). miR?141 expression file of GSE71533 which including 36 pancreatic cancer samples and 16 normal samples was downloaded from Gene Expression Omnibus(GEO)to analyze the expression of miR?141. Western blot was used to detect the expression of Bmi?1 in normal pancreatic cell and four pancreatic cancer cells Luciferase reporter assay was performed to analyze the relationship between Bmi?1 and miR?141. The effects of miR?141 and Bmi?1 on cell proliferation was examined by CCK8 and colony formation assay,respectively. Results:The qRT?PCR results showed that miR?141 was significantly down?regulated in pancreatic cancer cells. miR?141 was down?regulated in pancreatic cancer tissues by analyzing the microarray of GSE71533. Western blot analysis results demonstrated that Bmi?1 was up?regulated in pancreatic cancer cells. The dual luciferase assay indicated that miR?141 was anchored at the 3′?untranslated region of Bmi?1. Down?regulation of Bmi?1 inhibited the proliferation of pancreatic cancer cells. Upregulating miR?141 decreased the protein level of Bmi?1,thus repressing cell proliferation in pancreatic cancer cell. Conclusions:miR?141 acts as a tumor?suppressor in pancreatic cancer by targeting Bmi?1. These findings revealed that miR?141’s potential function in the treatment of pancreatic cancer.
查看全文   查看/发表评论  下载PDF阅读器