文章摘要
徐璐丹,樊 腾,张红伟,杨明月,马闻苛,秦鹏宇,岳修勤.不同剂量右美托咪定预处理对大鼠局灶性脑缺血再灌注损伤的影响[J].南京医科大学学报,2018,(7):973~977
不同剂量右美托咪定预处理对大鼠局灶性脑缺血再灌注损伤的影响
Effects of different doses of dexmedetomidine preconditioning on focal cerebral ischemia reperfusion injury in rats
投稿时间:2018-03-09  
DOI:10.7655/NYDXBNS20180719
中文关键词: 右美托咪定  脑缺血  再灌注损伤  S100β  细胞凋亡
英文关键词: dexmedetomidine  cerebral ischemia  reperfusion injury  S100β  apoptosis
基金项目:河南省卫生科技创新型人才工程中青年科技创新人才基金资助项目(20114155);河南省高等学校重点科研项目(18B310020)
作者单位
徐璐丹 新乡医学院第一附属医院麻醉科河南 新乡 453100 
樊 腾 新乡医学院第一附属医院麻醉科河南 新乡 453100 
张红伟 新乡医学院第一附属医院麻醉科河南 新乡 453100 
杨明月 新乡医学院第一附属医院麻醉科河南 新乡 453100 
马闻苛 新乡医学院第一附属医院麻醉科河南 新乡 453100 
秦鹏宇 新乡医学院第一附属医院麻醉科河南 新乡 453100 
岳修勤 新乡医学院第一附属医院麻醉科河南 新乡 453100 
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中文摘要:
      目的:观察不同剂量右美托咪定(dexmedetomidine,DEX)预处理对大鼠局灶性脑缺血再灌注损伤的影响。方法:50只健康雄性SD大鼠,随机分为假手术组(Sham组)、缺血再灌注组(I/R组)、小剂量右美托咪定组(D1组)、中等剂量右美托咪定组(D2组)、较大剂量右美托咪定组(D3组)。Sham组仅行外科操作而不造成缺血,Sham组、I/R组于外科操作前30 min腹腔注射生理盐水1 mL,D1、D2、D3组分别于缺血操作前30 min腹腔注射DEX 50、100、200 μg/kg。各组分别于术后24、48 h检测S100β蛋白含量,术后48 h测定Bcl?2、Bax含量及脑梗死体积比。结果:与Sham组相比,D1、D2、D3组S100β、Bax蛋白含量及脑梗死体积比均升高(P<0.05),Bcl?2含量降低(P<0.05);与I/R组相比,D1、D2、D3组S100β、Bax蛋白含量及脑梗死体积比均降低(P<0.05),Bcl?2含量升高(P<0.05);D1、D2、D3组间相比,D3组Bcl?2含量较D1、D2组降低(P<0.05),术后24 h S100β蛋白含量较D1、D2组升高(P<0.05),D1、D2组间差异无统计学意义;D1、D2、D3组间术后48 h S100β、Bax蛋白含量及脑梗死体积比差异无统计学意义。结论:右美托咪定预处理对局灶性脑缺血再灌注损伤大鼠有神经保护作用,其机制可能与抑制细胞凋亡有关,此作用在50和100 μg/kg间无明显差异。
英文摘要:
      Objective:To explore the effects of different doses of dexmedetomidine(DEX)preconditioning on focal in cerebral ischemia reperfusion injury rats. Methods:Fifty healthy male Sprague?Dawley rats were randomly divided into the sham operation group(sham group),the ischemia?reperfusion group(I/R group),the low?dose dexmedetomidine group(D1 group),the medium?dose dexmedetomidine group(D2 group),and the larger?dose dexmedetomidine group(D3 group). The sham group received surgical procedures without causing ischemia. The sham group and the I/R group received 1 mL normal saline intraperitoneally 30 minutes before ischemia,and the D1,D2 and D3 groups were injected with dexmedetomidine in 50 μg/kg,100 μg/kg and 200 μg/kg,respectively. The levels of S100β protein were measured 24 h and 48 h after reperfusion,the contents of Bcl?2,Bax and the volume of cerebral infarction were determined 48 h after reperfusion. Results:Compared with the I/R group,the protein contents of S100β,Bax,and the volume of cerebral infarction in the D1,D2 and D3 groups were lower(P<0.05),but were higher than those in the sham group(P<0.05);the Bcl?2 expression were increased(P<0.05),but were lower than that in the sham group(P<0.05). The content of Bcl?2 was lower and the content of S100β protein at 24 h after surgery was higher in the D3 group than that in the D1 and D2 groups(P<0.05),but were not statistically different between the D1 and D2 groups. The expression level of protein S100β,Bax and the volume of cerebral infarction were not statistically different between the D1,D2 and D3 groups. Conclusion:Dexmedetomidine preconditioning has neuroprotective effect on focal cerebral ischemia reperfusion injury in rats,and the mechanism may be related to the inhibition of apoptosis. The effect of dexmedetomidine has no significant difference between 50 μg/kg and 100 μg/kg.
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