文章摘要
李 琪,谷庆炜,曹 欣,李 倩.糖尿病小鼠胰腺组织Netrin⁃1表达及意义[J].南京医科大学学报,2018,(8):1039~1042,1053
糖尿病小鼠胰腺组织Netrin⁃1表达及意义
Expression and significance of Netrin⁃1 in pancreatic tissue in diabetic mice
投稿时间:2018-02-21  
DOI:10.7655/NYDXBNS20180805
中文关键词: 2型糖尿病  胰腺  Netrin⁃1  血糖  ins⁃1 细胞
英文关键词: type 2 diabetes mellitus  pancreatic tissue  Netrin⁃1  blood glucose  ins⁃1 cell
基金项目:国家自然科学基金(81200594);江苏省自然基金项目(BK20171121);南京市医学科技发展项目(ZKX14037)
作者单位
李 琪 南京医科大学附属南京医院内分泌科江苏 南京 210006 
谷庆炜 南京医科大学附属南京医院内分泌科江苏 南京 210006 
曹 欣 南京医科大学附属南京医院内分泌科江苏 南京 210006 
李 倩 南京医科大学附属南京医院内分泌科江苏 南京 210006 
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中文摘要:
      目的:研究糖尿病小鼠胰腺组织中Netrin?1的表达并探讨其在糖尿病发生过程中的作用。方法:C57BL/6J小鼠随机分为正常对照组和糖尿病模型组,应用RT?PCR和Western blot方法分别检测小鼠胰腺组织中Netrin?1 mRNA和蛋白的变化;在正常和异常葡萄糖浓度下培养大鼠胰岛细胞瘤细胞(ins?1),MTT检测细胞活性,RT?PCR和Western blot方法分别测定Netrin?1 mRNA和蛋白的表达情况。结果:糖尿病小鼠胰腺组织内Netrin?1的mRNA和蛋白表达增加,高浓度葡萄糖刺激ins?1细胞上调Netrin?1 mRNA和蛋白的表达。结论:糖尿病小鼠胰腺组织表达Netrin?1的水平增加,这与血糖异常相关,Netrin?1可能与糖尿病的发生发展相关。
英文摘要:
      Objective:To study the expression of Netrin?1 in pancreatic tissue in diabetic mice and explore the significance in the development of type 2 diabetes mellitus. Methods:C57BL/6J mice were divided into the normal group and the diabetic model group. RT?PCR and Western blot were used to detect the mRNA and protein expression of Netrin?1 in pancreatic tissue in mice;Ins?1 cells was cultured in medium with normal and abnormal concentration of glucose. MTT was used to detect the cell viability. RT?PCR and Western blot were used to detect the mRNA and protein of Netrin?1 respectively. Results:The mRNA and protein expression of Netrin?1 increased in pancreatic tissue of diabetic mice. While high concentration of glucose up?regulated the mRNA and protein expression of Netrin?1 in ins?1 cells. Conclusion:The level of Netrin?1 increases in pancreatic tissue in diabetic mice which correlates with the abnormal concentration of glucose. Netrin?1 may participate in the pathophysiology of diabetes.
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