文章摘要
陈爱东,魏 磊,刘胜平,陈亦江.miR⁃939对非小细胞肺癌侵袭能力及上皮细胞⁃间充质转化过程的影响[J].南京医科大学学报,2018,(8):1054~1058
miR⁃939对非小细胞肺癌侵袭能力及上皮细胞⁃间充质转化过程的影响
Influence of miR⁃939 on NSCLC cell invasion and EMT process
投稿时间:2018-01-17  
DOI:10.7655/NYDXBNS20180808
中文关键词: miRNAs  NSCLC  EMT  侵袭
英文关键词: miRNAs  NSCLC  EMT  invasion
基金项目:国家自然科学基金(981572263)
作者单位
陈爱东 南京医科大学第一附属医院胸心外科江苏 南京 210029 
魏 磊 南京医科大学第一附属医院胸心外科江苏 南京 210029 
刘胜平 南京医科大学第一附属医院胸心外科江苏 南京 210029 
陈亦江 南京医科大学第一附属医院胸心外科江苏 南京 210029 
摘要点击次数: 331
全文下载次数: 361
中文摘要:
      目的:探索miR?939对非小细胞肺癌的侵袭能力及上皮细胞?间充质转化过程(epithelial?mesenchymal transition,EMT)的影响。方法:实时聚合酶链反应(qRT?PCR)检测miR?939在非小细胞肺癌组织中的表达。Transwell侵袭实验分析miR?939对细胞侵袭能力的影响。通过Western blot检测EMT相关标志物的表达水平。结果:与癌旁组织相比,miR?939在NSCLC组织中的表达水平明显升高,且与TNM分期和淋巴结转移密切相关;同时,与正常肺上皮细胞相比,miR?939在NSCLC细胞系中的表达也显著升高。下调miR?939表达后,细胞侵袭能力明显降低(P <0.05)。另外,miR?939下调后可使EMT相关标志物E?cadherin升高,Vimentin降低。结论:miR?939可能作为不良因子,促进非小细胞肺癌侵袭转移,从而影响肿瘤发展。
英文摘要:
      Objective:The purpose of this study was to examine the expression and biological role of miR?939 in human non?small cell lung cancer(NSCLC)and epithelial?mesenchymal transition(EMT)progress. Methods:Quantitative real?time polymerase chain reaction(qRT?PCR)was used to detect the expression of miR?939 in NSCLC. Transwell analysis was used to determine the effect of miR?939 on cell invasion. EMT?related markers were detected by Western blot. Results:The expression of miR?939 in NSCLC tissues was significantly higher than that in adjacent non?cancerous tissues,and closely related to TNM staging and lymph node metastasis. Meanwhile,compared with normal lung epithelial cells,the expression of miR?939 in NSCLC cell lines was also significantly increased. The invasive ability of miR?939 cells was obviously decreased after down?regulation of miR?939 expression(P <0.05). In addition,miR?939 down?regulation caused E?cadherin increasing and Vimentin decreasing. Conclusion:miR?939 may serve as an adverse factor to promote cell invasion,thus affecting the development of NSCLC.
查看全文   查看/发表评论  下载PDF阅读器
关闭