Effect of electronic respiratory chain on the development of early female germ cells
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摘要:
目的：观察阻断电子呼吸链对早期雌性生殖细胞发育的影响。方法：取12.0 dpc（days postcoitum，交配后天数）雌性小鼠生殖嵴进行体外培养，经抗霉素A（antimycin A，AMA）作用后运用免疫荧光技术分别检测正常组、对照组以及实验组生殖细胞相关的分子标记物：DDX4（DEAD Asp-Glu-Ala-Asp box polypeptide 4）、γ-H2AX（the phosphorylated form of Histone H2AX）、OCT4（POU domain，class 5，transcription factor 1）及STRA8（stimulated by retinoic acid gene 8）的表达，试剂盒检测ATP和ROS含量，观察阻断电子呼吸链对早期雌性生殖细胞发育的影响。结果：AMA作用后雌性生殖嵴发育明显阻滞。进一步研究提示AMA处理后导致三磷酸腺苷（adenosine triphosphate，ATP）明显下降而活性氧（reactive oxygen species，ROS）显著增加，从而可能导致减数分裂起始异常。结论：电子呼吸链与雌性小鼠生殖嵴的发育关系密切。
Abstract:
Objective：To observe the effect of blocking electronic respiratory chain on the development of early female germ cells. Methods：We labeled normal，control and treatment group with germ cells associated markers：DDX4（DEAD Asp-Glu-Ala-Asp box polypeptide 4），γ-H2AX（the phosphorylated form of Histone H2AX），OCT4（POU domain，class 5，transcription factor 1）and STRA8（stimulated by retinoic acid gene 8） to assay the effects of the early female germ cells after blocking the electron respiratory chain by antimycin A（AMA）. Results：12.0 dpc（days postcoitum）female mice gonads were cultured in vitro，and we found aberrant gonadogenesis after treatment with AMA，showing obviously decreased ATP（adenosine triphosphate）and increased ROS（reactive oxygen species），thus，leading to the abnormal meiosis initiation. Conclusion：The electron respiratory chain was highly linked with the gonads development in female mice.
