Objective：To explore the standard method of lower limbs ischemia model establishment in rabbits to provide a consistent condition for the subsequent study on the basis of ischemia. Methods：Twelve New Zealand rabbits were divided into 2 groups（Group I and Group F）according to a random number table with 6 rabbits in each. The rabbits were ligated at the lower segment of the left common iliac artery（cross of internal and external iliac artery）in the group I，or at the start level of left femoral artery（inguinal ligament）in the group F，respectively，followed by DSA to observe the lower limbs blood supply，and measurement of vastus medial shell temperature at the 3rd and 10th day of ligation. Results：No blood supply of branch artery was observed in group I whereas there were still branches from the external iliac artery to the lower limb（P < 0.05）. Conclusion：Ligation at the lower segment of the common iliac artery can promise to block the blood supply for the lower limb，which can be used to establish a consistent lower limb ischemia model.