转基因Tomoregulin⁃1低表达加速扩张型心肌病小鼠的病理进程
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

南京医科大学科技发展基金重点项目(2016NJMUZD019)


Transgenic low expression of Tomoregulin⁃1 accelerated dilated cardiomyopathy patholo⁃gical progress in mice
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探究Tomoregulin-1对扩张型心肌病(dilated cardiomyopathy,DCM)小鼠病理进程的影响。方法:Western blot方法检测Tomoregulin-1在cTnTR141W DCM小鼠模型心脏组织中的表达水平;于2、4和6月龄对同窝阴性(non-transgenic littermates,NTG)小鼠、心脏组织特异Tomoregulin-1低表达小鼠、cTnTR141W DCM小鼠和心脏组织特异Tomoregulin-1低表达×cTnTR141W双转基因(double transgenic,DTG)小鼠进行M型超声心动图和病理组织学表型分析。结果:Tomoregulin-1在cTnTR141W DCM小鼠模型心脏组织中表达显著增高;M型超声心动图显示,与NTG小鼠相比,心脏组织特异Tomoregulin-1低表达小鼠在2、4和6月龄时心脏均呈现显著的室壁变薄、心腔增大和心收缩功能减退的表型;与cTnTR141W DCM模型小鼠相比,DTG小鼠在2、4和6月龄时心脏均呈现显著的室壁变薄的表型,亦呈现心腔增大和心收缩功能减退的趋势;病理组织学显示,与NTG小鼠相比,心脏组织特异Tomoregulin-1低表达小鼠心肌细胞排列紊乱并出现间质纤维化的表型;与cTnTR141W DCM模型小鼠相比,DTG小鼠的心肌细胞排列紊乱和间质纤维化程度更加严重。结论:转基因低表达Tomoregulin-1加速了cTnTR141W DCM小鼠的病理进程,Tomoregulin-1可能是DCM调节的重要修饰基因。

    Abstract:

    Objective:To explore the effects of Tomoregulin-1 on dilated cardiomyopathy(DCM)pathological progress in mice. Methods:The expression level of Tomoregulin-1 in the heart of wild-type and cTnTR141W DCM transgenic mice was detected by Western blot. The phenotype analysis of the non-transgenic littermates(NTG),heart-specific Tomoregulin-1 knockdown(Tomoregulin-1-kd),cTnTR141W and heart-specific Tomoregulin-1-kd×cTnTR141W double transgenic(DTG) mice at 2,4 and 6 months of age were analyzed by M-mode echocardiography and histopathologic examination. Results:The expression of Tomoregulin-1 was significantly increased in the heart of cTnTR141W DCM transgenic mice. M-mode echocardiography showed that the heart-specific Tomoregulin-1-kd mice presented thin-walled ventricles,larger left ventricular diameters and decreased cardiac function at 2,4 and 6 months of age compared with the NTG mice. The DTG mice presented significantly thin-wall ventricles and the tendency of larger left ventricular diameters and decreased cardiac function at 2,4 and 6 months of age compared with the cTnTR141W DCM mice. Myocardial disarray and fibrosis were clearly observed in the heart tissues from the Tomoregulin-1-kd mice compared with the NTG mice. The DTG mice presented more serious pathological phenotype compared with the cTnTR141W DCM mice. Conclusion:Transgenic low expression of Tomoregulin-1 accelerated DCM pathological progress in mice. Tomoregulin-1 may be an important modifier gene of DCM.

    参考文献
    相似文献
    引证文献
引用本文

郑 媛,侯道荣,付鹤玲,尹 媛,鲍 丹.转基因Tomoregulin⁃1低表达加速扩张型心肌病小鼠的病理进程[J].南京医科大学学报(自然科学版),2018,(9):1187-1191

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-07-03
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-09-17
  • 出版日期: