文章摘要
丁 叶,任甲翠,徐 进,秦 蕊,汪之顼.Salubrinal对高糖高脂饲料诱导的妊娠期糖尿病小鼠的作用[J].南京医科大学学报,2018,(9):1192~1197
Salubrinal对高糖高脂饲料诱导的妊娠期糖尿病小鼠的作用
The effect of salubrinal on gestational diabetic mice induced by high⁃sucrose/high⁃fat diet
投稿时间:2017-08-21  
DOI:10.7655/NYDXBNS20180904
中文关键词: Salubrinal  妊娠期糖尿病  胰岛β细胞  高糖高脂饲料  小鼠
英文关键词: salubrinal  gestational diabetes mellitus  pancreatic β cells  high⁃sucrose/high⁃fat diet  mouse
基金项目:江苏省高校自然科学研究面上项目(15KJB330003);国家自然科学基金(81502802);江苏高校优势学科建设工程资助项目(公共卫生与预防医学)
作者单位
丁 叶 南京医科大学公共卫生学院儿少卫生与妇幼保健学系江苏 南京 211166 
任甲翠 南京医科大学公共卫生学院儿少卫生与妇幼保健学系江苏 南京 211166 
徐 进 南京医科大学公共卫生学院儿少卫生与妇幼保健学系江苏 南京 211166 
秦 蕊 南京医科大学公共卫生学院儿少卫生与妇幼保健学系江苏 南京 211166 
汪之顼 南京医科大学公共卫生学院儿少卫生与妇幼保健学系江苏 南京 211166 
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中文摘要:
      目的:探讨Salubrinal对高糖高脂饲料诱导的妊娠期糖尿病(gestational diabetes mellitus,GDM)小鼠能量摄入量、体重、血糖、胰岛素和胰岛形态学变化的影响。方法:C57BL/6J雌性小鼠随机分为:正常对照组(C组)、GDM模型组(G组)和GDM Salubrinal干预组(G+S组);多个时间点测量小鼠能量摄入量、体重和血糖水平;孕第10天收集血液测定胰岛素水平,计算胰岛β细胞功能指数(HOMA?B)和胰岛素抵抗指数(HOMA?IR);孕第17天处死小鼠,进行胰岛形态学观察。结果:G组、G+S组在多个时间点能量摄入量和体重均大于C组;而G组和G+S组之间的差异不大。在交配前和孕第10天,血糖水平均表现为G+S组>G组>C组;在孕第17天,3组之间差异不大。在孕第10天,3组的胰岛素水平差异不大,但HOMA?B值表现为G+S组<G组<C组,HOMA?IR值表现为G+S组>G组>C组。胰岛形态学检查发现,C组胰岛形态和胰岛β细胞超微结构较正常;G组和G+S组胰岛数量均增多,胰岛增生和萎缩同时存在,胰岛β细胞超微结构异常,线粒体严重肿胀,粗面内质网高度膨胀。结论:Salubrinal对高糖高脂饲料诱导的GDM小鼠不具有防治潜力。
英文摘要:
      Objective:The present study focuses on the effect of salubrinal on energy intake,body weight,blood glucose,serum insulin and morphological changes of pancreatic islets in gestational diabetic mice induced by high?sucrose/high?fat diet. Methods:Female C57BL/6J mice were divided into three groups randomly:normal control group(C),gestational diabetic model group(G)and salubrinal intervention group(G+S). Energy intake,body weight,food intake and blood glucose were measured at multiple time points before and during pregnancy. At the 10th day of pregnancy,the blood was collected for the measurement of serum insulin levels. Then the scores of pancreatic β?cell function(HOMA?B)and insulin resistance(HOMA?IR)were calculated. At the 17th day of pregnancy,the mice were sacrificed,and the pancreas was collected for morphological examination. Results:The energy intake and body weight of group G and group G+S were respectively higher than that of group C at multiple time points before and during pregnancy,whereas there was little difference in these two parameters between group G and group G+S. After 1?month feeding,the tendency of blood glucose level was showed as:group G+S > group G > group C,and this tendency was more significant at the 10th day of pregnancy. However,there was no significant difference in blood glucose among the 3 groups at the 17th day of pregnancy. Although there was little difference in serum insulin levels among the 3 groups at the 10th day of pregnancy,HOMA?B showed:group G+S < group G < group C,and HOMA?IR showed:group G+S > group G > group C. Morphological examination showed that the morphology of pancreatic islets and the ultrastructure of pancreatic β cells in group C were normal. However,the number of pancreatic islets increased in group G and group G+S. The hyperplasia and atrophy of pancreatic islets occurred simultaneously in these 2 groups. Meanwhile,the abnormal ultrastructure of pancreatic β cells was characterized by severe swollen mitochondria and highly expansion of rough endoplasmic reticulum. Conclusion:These findings suggest that salubrinal has no potential for preventing and controlling gestational diabetic mice induced by high?sucrose/high?fat diet.
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