文章摘要
夏 鑫,靳建亮,左国平,任子健.Bmi⁃1通过下调NF⁃κB信号通路防治小鼠萎缩性胃炎[J].南京医科大学学报,2018,(9):1208~1211
Bmi⁃1通过下调NF⁃κB信号通路防治小鼠萎缩性胃炎
Bmi⁃1 protects mice from atrophic gastritis by down⁃regulating NF⁃κB signaling pathway
投稿时间:2017-12-19  
DOI:10.7655/NYDXBNS20180907
中文关键词: Bmi⁃1  基因敲除小鼠  萎缩性胃炎  NF⁃κB信号通路
英文关键词: Bmi⁃1  gene knock⁃out mice  atrophic gastritis  NF⁃κB signaling pathway
基金项目:国家自然科学基金(81571371)
作者单位
夏 鑫 南京医科大学人体解剖学系江苏 南京 211166 
靳建亮 南京医科大学人体解剖学系江苏 南京 211166 
左国平 南京医科大学人体解剖学系江苏 南京 211166 
任子健 南京医科大学人体解剖学系江苏 南京 211166 
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中文摘要:
      目的:明确Bmi?1是否有防治萎缩性胃炎的作用及其机制。方法:针对7周龄Bmi?1基因缺失(Bmi?1 knock out,BKO)纯合子小鼠和同窝野生型(wild type,WT)小鼠,利用组织学切片、免疫组织化学和 Western blot比较分析胃的表型差异。结果:与WT小鼠相比,BKO小鼠表现为胃壁变薄、腺体萎缩和炎症浸润的萎缩性胃炎表型。免疫组化结果显示白细胞介素6(interleukin 6,IL?6)、肿瘤坏死因子α(tumor necrosis factor α,TNF?α)阳性细胞面积和核因子?κB?p65(nuclear factor?κB?p65,NF?κB?p65)阳性细胞数明显增加;Western blot 结果显示IL?6、TNF?α和NF?κB?p65蛋白表达水平明显升高。结论:在小鼠中,Bmi?1可通过下调NF?κB信号通路防治萎缩性胃炎。
英文摘要:
      Objective:To investigate the role of Bmi?1 in preventing atrophic gastritis in mice. Methods:The phenotype of stomach was compared between wild?type and Bmi?1 gene knock?out mice at 7 weeks of ages by histology,immunohistochemistry and Western?blot. Results:Bmi?1 gene knock?out mice displayed thin gastric wall,inflammatory infiltration in gastric mucosa and atrophy of gastric gland. The expression of interleukin?6(IL?6),tumor necrosis factor α(TNF?α) and nuclear factor?κB?p65(NF?κB?p65) is up?regulated in Bmi?1 knock?out mice. Conclusion:Bmi?1 could protect mice from atrophic gastritis by down?regulating NF?κB signaling pathway.
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