文章摘要
孟亚奇,贾 嫚,姜 洁,杨 翌,姚 欣.IL⁃36γ在哮喘患者血清及支气管上皮细胞中的表达及意义[J].南京医科大学学报,2018,(9):1264~1268,1274
IL⁃36γ在哮喘患者血清及支气管上皮细胞中的表达及意义
The expression and significance of IL⁃36γ in serum and bronchial epithelial cells of asthma patients
投稿时间:2018-03-23  
DOI:10.7655/NYDXBNS20180918
中文关键词: 支气管哮喘  IL⁃36γ  支气管上皮细胞
英文关键词: asthma  IL⁃36γ  bronchial epithelial cell
基金项目:国家自然科学基金(81070025);江苏省医学重点人才项目(ZDRCA2016020)
作者单位
孟亚奇 南京医科大学第一附属医院呼吸与危重症医学科江苏 南京 210029 
贾 嫚 南京医科大学第一附属医院呼吸与危重症医学科江苏 南京 210029 
姜 洁 南京医科大学第一附属医院呼吸与危重症医学科江苏 南京 210029 
杨 翌 南京医科大学第一附属医院呼吸与危重症医学科江苏 南京 210029 
姚 欣 南京医科大学第一附属医院呼吸与危重症医学科江苏 南京 210029 
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中文摘要:
      目的:研究白细胞介素(interleukin,IL)?36γ在支气管哮喘患者血清中的水平及在支气管上皮细胞中的表达,探讨其在哮喘病情评估中的潜在临床价值。方法:收集24例哮喘患者及24例同期健康志愿者的临床资料及血清标本,采用酶联免疫吸附实验检测血清IL?36γ表达水平,分析其与肺功能、病情控制水平以及血清IL?13的相关性。采用实时定量PCR法检测人支气管上皮细胞16HBE IL?36γ的mRNA表达以及相关炎症因子对该过程的影响。结果:哮喘组血清IL?36γ表达水平高于健康对照组[(175.90 ± 25.70)pg/mL vs.(100.40 ± 8.49)pg/mL,P=0.008],其表达与肺功能FEV1/FVC(r=-0.347,P=0.016)、FEV1%pred(r=-0.454,P=0.001)均呈负相关,与血清IL?13水平(r=0.611,P=0.003)呈正相关。根据哮喘患者的病情控制水平进行分级,血清IL?36γ主要在未控制组高表达,明显高于部分控制组(P < 0.05)、完全控制组(P < 0.05)及健康对照组(P < 0.001)。外源重组蛋白IL?13刺激16HBE促进IL?36γ mRNA呈浓度和时间依赖性表达。结论:哮喘患者血清IL?36γ表达增高,其表达上调可能和IL?13有关,该蛋白可能是哮喘评估和病情监测的潜在生物学标志物。
英文摘要:
      Objective:To investigate the expression and clinical significance of interleukin(IL)?36γ in serum and bronchial epithelial cells of asthma patients. Methods:The levels of serum IL?36γ of 24 cases of asthma patients and 24 cases of healthy volunteers were determined by enzyme?linked immunosorbent assay(ELISA). The effects of asthma?related inflammatory mediators on IL?36γ expression were examined by real time quantitative PCR in human bronchial epithelial cell line(16HBE). Results:The levels of serum IL?36γ were significantly increased in asthma patients compared with healthy controls[(175.90 ±25.70)pg/mL vs.(100.40 ± 8.49)pg/mL,P=0.008]. Specifically,The increased levels of serum IL?36γ were negatively correlated with the reduced lung function including FEV1/FVC(r=-0.347,P=0.016)and FEV1% pred(r=-0.454,P=0.001)and had a positive correlation with serum IL?13 levels(r=0.611,P=0.003). Moreover,IL?36γ expression was highest in uncontrolled group of asthma patients than those without asthma(P < 0.001)or those with well to partly controlled asthma(P < 0.05). In vitro,IL?13 treatment induced a dose and time?dependent up?regulation of IL?36γ mRNA expression in 16HBE. Conclusion:Serum IL?36γ levels are elevated in asthma patients compared with healthy control subjects. Serum IL?36γ may be a potential novel circulating biomarker for the disease assessment and monitoring of asthma.
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