文章摘要
杨妍婷,赵苏瑛,张亚楠,袁葛惠,曹淑源,郭文慧,王 丽,张 展,李 磊,吴 倩.基于电感耦合等离子体质谱法对血流感染患者血清中相关元素的研究[J].南京医科大学学报,2018,(11):1637~1642
基于电感耦合等离子体质谱法对血流感染患者血清中相关元素的研究
Determination of related elements in the serum of patients with bloodstream infection by inductively coupled plasma mass spectrometry
投稿时间:2017-09-04  
DOI:10.7655/NYDXBNS20181136
中文关键词: 电感耦合等离子体质谱  血流感染  大肠杆菌  金属元素  金属组学
英文关键词: inductively coupled plasma mass spectrometry  bloodstream infection  E. coli  metal elements  metallomics
基金项目:国家自然科学基金(81673228);江苏省高校品牌专业建设工程资助项目(PPZY2015A067)
作者单位
杨妍婷 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
赵苏瑛 南京中医药大学附属医院检验科江苏 南京 210029 
张亚楠 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
袁葛惠 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
曹淑源 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
郭文慧 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
王 丽 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
张 展 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
李 磊 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
吴 倩 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
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中文摘要:
      目的:以大肠杆菌血流感染者为例,基于电感耦合等离子体质谱(inductively coupled plasma mass spectrometry,ICP?MS)方法建立血流感染患者血清中金属元素谱,并筛选关键金属元素,为早期诊断及治疗血流感染提供依据。方法:以6Li、89Y、45Sc、115In、159Tb和209Bi做内标,采用ICP?MS法测定199例正常人血清中68种金属元素,建立元素谱;同时基于金属组学对大肠杆菌感染者和健康对照者做进一步研究,运用主成分分析和偏最小二乘判别分析等进行多元统计分析,筛选出差异性元素。结果:基于ICP?MS建立了血流感染患者及健康人群血清中的金属元素谱,筛选出大肠杆菌血流感染者与健康对照者血清中的差异性元素Zn、Ce、Pd、Cu、Sn、Se、Ba、Au和Fe。结论:采用ICP?MS可以快速高通量地检测并建立人血清中的金属元素谱;筛选出大肠杆菌血流感染患者和健康对照者血清中的差异性元素,为从金属组学角度诊断、预防和治疗血流细菌感染提供研究基础。
英文摘要:
      Objective:To establish a method for the determination of elements in serum of patients with bloodstream infection by inductively coupled plasma mass spectrometry(ICP?MS). Combined with metallomics,we obtained the metal element profile of E. coli bloodstream infection(BSI)and screened key element markers,which provides proof for diagnosis and prevention of bloodstream infection. Methods:Taking 6Li,89Y,45Sc,115In,159Tb,and 209Bi as internal standards,ICP?MS was used to measure 68 kinds of metal elements in serum of 199 normal humans,diluting serum samples and internal standard method for correction,with kinetic energy discrimination to eliminate mass spectroscopic interference. Meanwhile,68 kinds of metal elements in serum from 19 BSI patients with E. coli infection and 19 healthy people were tested by ICP?MS. Statistical analysis was performed using principal components analysis(PCA)and partial least squares discriminate analysis(PLS?DA),and the differences were analyzed. Results:Based on ICP?MS,the profile of metal elements in blood serum of patients with BSI and healthy people was established,and the differential elements Zn,Ce,Pd,Cu,Sn,Se,Ba,Au and Fe in the serum of the patients with BSI and healthy controls were screened out. Conclusion:We established multi?elementary metal profiles using ICP?MS in human serum,which is acceptable for a clinical routine analysis. There were differences in the serum elements between BSI patients and the healthy controls. The results provide a basis for the diagnosis,prevention and treatment of blood bacterial infections from the perspective of metallomics.
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