文章摘要
周 明,黄君文,陈 婷,宋佳成,马占龙.Tenascin⁃X蛋白及CD34在动脉粥样斑块形成过程中的表达及相关性的实验研究[J].南京医科大学学报,2019,(2):181~186
Tenascin⁃X蛋白及CD34在动脉粥样斑块形成过程中的表达及相关性的实验研究
Expression and correlation of tenascin⁃X and CD34 in atherosclerotic plaque formation
投稿时间:2018-05-30  
DOI:10.7655/NYDXBNS20190204
中文关键词: 动脉粥样硬化  ApoE基因敲除小鼠  Tenascin⁃X  血管形成
英文关键词: atherosclerosis  ApoE gene knocked out mice  Tenascin⁃X  angiogenesis
基金项目:国家自然科学基金面上项目(81471723)
作者单位
周 明 南京医科大学第一附属医院影像科江苏 南京 210029 
黄君文 南京医科大学第一附属医院影像科江苏 南京 210029 
陈 婷 南京医科大学第一附属医院影像科江苏 南京 210029 
宋佳成 南京医科大学第一附属医院影像科江苏 南京 210029 
马占龙 南京医科大学第一附属医院影像科江苏 南京 210029 
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中文摘要:
      目的:检测肌腱糖蛋?X(Tenascin?X,TNX)与血管内皮细胞标志物CD34在ApoE基因敲除小鼠(apolipoprotein E gene knock out mice,ApoE-/-mice)动脉粥样硬化斑块形成过程中的动态表达及探讨两者的相关性。方法:高脂饲养ApoE-/-小鼠建立动脉粥样硬化动物模型为实验组,普通饲料喂养的C57BL/6小鼠为对照组。分别于喂养第8、12、20、28、36周时测量血脂水平,采集主动脉血管标本,油红O染色和HE染色观察主动脉的病理学改变并进行计量组织学及免疫组化分析。结果:实验组低密度脂蛋白(low?density lipoprotein,LDL)、总胆固醇(total cholesterol,TC)水平在各期中均高于对照组(P < 0.05)。随着造模时间的延长,实验组斑块面积及斑块面积/管腔面积比值不断增加(斑块面积:F=31.473,P < 0.001;斑块面积/管腔面积:F=178.422,P < 0.001)。斑块内TNX与CD34的表达量随着饲养时间的增加而增多,两者均在36周时表达最多,并且TNX与CD34阳性微血管均在纤维帽及脂质核心周围高表达且呈正相关(r=0.87,P < 0.001)。而对照组中未见明显斑块形成。结论:Tenascin?X和CD34的表达量随着斑块进展而增多,且两者在斑块中的表达区域相似。TNX可能与斑块稳定性密切相关。
英文摘要:
      Objective:To detect dynamic expression of Tenascin?X(TNX)and CD34(marker of vascular endothelial cell)and explore the correlation of both in formation of atherosclerotic plaque in apolipoprotein E gene knockout mice(ApoE-/-mice). Methods:The experimental group of atherosclerotic model was established in ApoE-/- mice fed with a high?fat diet,and the normal C57BL/6 mice fed with normal diet served as the control group. Blood lipid levels were measured and blood samples of aorta were collected at 8 th,12 th,20 th,28 th,and 36 th week after feeding respectively. Oil red O staining and HE staining were used to observe the pathological changes of the aorta,and the quantitative histological and immunohistochemical were analysed meanwhile. Results:The experimental group of low?density lipoprotein(LDL),total cholesterol(TC) levels in each period were higher than the control group(P < 0.05). With the extension of modeling time,the plaque area and plaque area/lumen area ratio were continually increased in experimental group(plaque area:F=31.473,P < 0.001;plaque area/lumen area:F=178.422,P < 0.001). The expression quantities of TNX and CD34 increased along with feeding time extendins,and both of them were most expressed at 36 weeks. The TNX and CD34 positive microvessels were highly expressed around the fibrous cap and lipid core,and both were positively correlated(r=0.87,P < 0.001). However,there were no significant formation of plaques seen in the control group. Conclusion:The expression of Tenascin?X and CD34 increased with the progress of plaque,and the expression sites of Tenascin?X and CD34 in plaque were similar. TNX may be closely related to plaque stability.
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