文章摘要
马 静,芮海波,陈全战,汪振炯,陈玉胜.灵芝多糖对链脲佐菌素诱导的糖尿病肾病小鼠抗炎活性及疗效研究[J].南京医科大学学报,2019,(3):326~331,337
灵芝多糖对链脲佐菌素诱导的糖尿病肾病小鼠抗炎活性及疗效研究
Study on anti⁃inflammatory and therapeutic properties of Ganoderma lucidum polysacch⁃arides on diabetic nephropathy in streptozotocin⁃induced mice
投稿时间:2018-04-12  
DOI:10.7655/NYDXBNS20190303
中文关键词: 灵芝多糖  糖尿病肾病  炎症小体  HE染色  Western blot
英文关键词: Ganoderma lucidum polysaccharides(GL⁃PS)  diabetic nephropathy  inflammasome  hematoxylin⁃eosin(HE)staining  Western blot
基金项目:国家自然科学基金(31471699);南京市属高校“十三五”重点学科建设项目(4217025)
作者单位
马 静 东南大学医学院附属南京同仁医院药学部江苏 南京 211102 
芮海波 南京市中医院药学部江苏 南京 210016 
陈全战 南京晓庄学院食品科学学院江苏 南京 211171 
汪振炯 南京晓庄学院食品科学学院江苏 南京 211171 
陈玉胜 南京晓庄学院食品科学学院江苏 南京 211171 
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中文摘要:
      目的:探讨灵芝多糖(Ganoderma lucidum polysaccharides,GL?PS)对链脲佐菌素(streptozotocin,STZ)诱导的糖尿病肾病小鼠的疗效及机制。方法:STZ 100 mg/(kg·d)腹腔注射诱导糖尿病肾病小鼠模型,并随机分为6组:正常组、模型组、模型给药组(50、100、200 mg/kg GL?PS)、阳性对照组[5 mg/kg替米沙坦(telmisartan,TMS)],每组10只,连续灌胃给药8周后,收集动物的尿液、血液和肾脏,并使用试剂盒检测相关指标。以苏木精?伊红(hematoxylin?eosin,HE)染色对肾组织进行病理学检验,Western blot法检测小鼠肾组织中NOD样蛋白3(NOD?like receptor 3,NLRP3)、核因子(nuclear factor,NF)?κB及 白介素(interleukin,IL)?1β的蛋白表达。结果:GL?PS和TMS显著降低STZ诱导的糖尿病肾病小鼠血清中尿素氮(blood urea nitrogen,BUN)、肌酐(serum creatinine,SCr)、尿酸(serum uric acid,SUA)、血糖(glucose,GLU)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)及白介素(interleukin,IL)?1β、IL?18、IL?6 和肿瘤坏死因子(tumor necrosis factor,TNF)?α的水平;显著提高高密度脂蛋白胆固醇(high?density lipoprotein cholesterol,HDLC)水平;24 h 尿蛋白排泄量亦明显较模型组降低;HE 染色及Western blot表明 GL?PS和TMS显著减轻炎症因子对肾组织的损伤,抑制NLRP3/NF?κB炎性小体的活化,下调IL?1β蛋白表达。结论:GL?PS对糖尿病肾病具有显著疗效,其机制可能与抑制炎症因子活化,改善肾功能有关。
英文摘要:
      Objective:To study the therapeutic properties and possible mechanism of Ganoderma lucidum polysaccharides(GL?PS)on diabetic nephropathy in streptozotocin(STZ)?induced mice. Methods:STZ[100 mg/(kg·d)] was administered by intraperitoneal injection to induce diabetic nephropathy model in mice. The mice were randomly divided into 6 groups(n=10 in each group):the normal group,model group,GL?PS groups with GL?PS dose of 50,100 and 200 mg/kg,and the telmisartan(TMS)group(5 mg/kg). After 8 continuous weeks of intragastric administration,the mice uric,blood and renal were collected,and some physiological and biochemical indexes were determined by kits. Renal pathology was examined by hematoxylin?eosin(HE)staining. Protein expressions of NOD?like receptor 3(NLRP3),nuclear factor(NF)?κB and interleukin(IL)?1β in renal tissues were also analyzed by Western blot. Results:Compared with the model group,GL?PS and TMS obviously decreased the levels of blood urea nitrogen(BUN),serum creatinine(SCr),serum uric acid(SUA),glucose(GLU),total cholesterol(TC),triglyceride(TG),and interleukin(IL)?1β,IL?18,IL?6,as well as tumor necrosis factor(TNF)?α and urinary protein contents excreted during 24 h;significantly increased the high?density lipoprotein cholesterol(HDLC)level in STZ?induced diabetic nephropathy mice. HE and Western blot results indicated that GL?PS and TMS also obviously alleviated renal inflammatory reaction,inhibited NLRP3/NF?κB inflammasome activation,and down?regulated the protein expression of IL?1β. Conclusion:GL?PS has remarkable therapeutic properties on diabetic nephropathy in mice. The possible mechanism may be related to suppressing of inflammasome activation and improving of renal function.
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