文章摘要
冯楚炎,王向明,屠甜甜,Deeraj Mungun,许婷婷,徐婉莹,郭 妍.远端缺血后适应通过抑制RAGE的表达实现对心肌的保护作用[J].南京医科大学学报,2019,(4):500~504
远端缺血后适应通过抑制RAGE的表达实现对心肌的保护作用
Remote ischemic postconditioning protects myocardium by inhibiting the expression of RAGE
投稿时间:2018-10-17  
DOI:10.7655/NYDXBNS20190407
中文关键词: 缺血再灌注  晚期糖基化终末产物受体  远端缺血后适应
英文关键词: ischemic reperfusion  advanced glycation end⁃product receptor  remote ischemic postconditioning
基金项目:江苏省干部保健科研课题(BJ18017)
作者单位
冯楚炎 南京医科大学第一附属医院老年心血管科江苏 南京 210029 
王向明 南京医科大学第一附属医院老年心血管科江苏 南京 210029 
屠甜甜 南京医科大学第一附属医院老年心血管科江苏 南京 210029 
Deeraj Mungun 南京医科大学第一附属医院老年心血管科江苏 南京 210029 
许婷婷 南京医科大学第一附属医院老年心血管科江苏 南京 210029 
徐婉莹 南京医科大学第一附属医院老年心血管科江苏 南京 210029 
郭 妍 南京医科大学第一附属医院老年心血管科江苏 南京 210029
江苏盛泽医院心血管科江苏 苏州 215228 
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中文摘要:
      目的:探讨晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)在远端缺血后适应改善心肌缺血再灌注中的作用。方法:将34只8~9周龄C57BL/6J小鼠随机分为6组:假手术组(Sham组)、FPS?ZM1对照组(FZM1组)、缺血再灌注组(IR组)、FPS?ZM1干预组(FZM1+IR组)、远端缺血后适应组(RIPostC+IR组)和FZM1+远端缺血后适应干预组(FZM1+RIPostC+IR组)。通过结扎左冠状动脉,建立小鼠心肌缺血再灌注模型。采用小鼠心超仪检测小鼠左室射血分数(left ventricular ejection fraction,LVEF)和左室缩短分数(left ventricular fractional shortening,LVFS),运用酶联免疫分析(ELISA)法和蛋白印迹法检测炎症因子白介素6(interleukin 6,IL?6)、核因子(nudear factor,NF)?κB P65蛋白、RAGE蛋白。结果:①与IR组相比,RIPostC能显著增加LVEF(P < 0.01)和LVFS(P < 0.01)。②与IR组相比,RIPostC能显著降低RAGE(P < 0.001)的表达。③与IR组相比,RIPostC能显著减少NF?κB P65(P < 0.001)和IL?6(P < 0.001)的表达。结论:RIPostC对心肌缺血再灌注损伤具有保护作用。该作用可能通过抑制RAGE的表达进而降低炎性反应而实现。
英文摘要:
      Objective:This study aimed to investigate the role of receptor for advanced glycation end products(RAGE)in improving myocardial ischemia and reperfusion after remote ischemic postconditioning. Methods:Thirty?four C57/B6 mice aged 8?9 weeks were randomly divided into six groups:the sham operation group(sham),the FPS?ZM1 control group(FZM1),the ischemia and resperfusion group(IR),the FPS?ZM1 intervention group(FZM1+IR),the RIPostC group(RIPostC+IR)and the RIPostC intervention group(RIPostC+FZM1+IR). The anterior descending coronary artery was ligated to create myocardial ischemia?reperfusion model of mouse. The left ventricular ejection fraction(LVEF)and left ventricular shortening fraction(LVFS)of mice were detected by mouse cardiac ultrasonography,and the inflammatory factor IL?6,NF?κB P65 protein and RAGE protein were detected by enzyme?linked immunoassay(ELISA)and Western blotting. Results:①RIPostC significantly increased LVEF(P < 0.01)and LVFS(P < 0.01)compared with the I/R group. ②Compared with the IR group,RIPostC significantly reduced the expression of RAGE(P < 0.001). ③RIPostC significantly reduced the expression of NF?κB P65(P < 0.001)and IL?6(P < 0.001)compared with the I/R group. Conclusion:RIPostC was effective in protecting against myocardial ischemia?reperfusion injury. The cardioprotective effects of RIPostC may be achieved directly by inhibiting the expression of RAGE and thereby reducing the inflammatory response.
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