文章摘要
刘亚文,陆亚倩,戴海峰,李 谨,王 娟.不同寡营养条件下饥饿期粪肠球菌基因转录水平的体外研究[J].南京医科大学学报,2019,(5):668~672
不同寡营养条件下饥饿期粪肠球菌基因转录水平的体外研究
In vitro study on the gene transcriptional level of Enterococcus faecalis during starvation phase under different oligotrophic conditions
投稿时间:2018-08-27  
DOI:10.7655/NYDXBNS20190508
中文关键词: 粪肠球菌  葡萄糖饥饿应激  转录组测序
英文关键词: enterococcus faecalis  glucose starvation stress  transcriptome sequencing
基金项目:国家自然科学基金(81300868);江苏省卫生厅科研课题资助(H201433);江苏省干部保健科研项目(BJ15030)
作者单位
刘亚文 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙体牙髓病科江苏 南京 210029 
陆亚倩 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙体牙髓病科江苏 南京 210029 
戴海峰 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙体牙髓病科江苏 南京 210029 
李 谨 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙体牙髓病科江苏 南京 210029 
王 娟 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙体牙髓病科江苏 南京 210029 
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中文摘要:
      目的:在体外模拟根管致病压力条件下,探究不同寡营养条件对饥饿期粪肠球菌基因转录水平的影响。方法:将粪肠球菌标准菌株(ATCC 29212)诱导至饥饿期后培养于不同寡营养条件下(0%、0.05%、0.15%和0.25%葡萄糖浓度的培养液),1周后对其基因转录水平及代谢通路进行分析。结果:转录组结果显示,与0.25%相比,0.15%葡萄糖浓度基因转录水平没有明显变化,0.05%及0%葡萄糖浓度基因转录水平变化明显,以0%葡萄糖浓度变化最为显著,在GO功能上,显著富集于代谢进程及催化活性,在KEGG功能上并无显著差异,排名前3的代谢通路有ABC转运器、磷酸转移酶系统、氨基酸生物合成。结论:寡营养条件下,粪肠球菌基因转录水平的变化可能和其在恶劣生存条件下仍能生存和致病有关,在本研究基础上将进一步探究粪肠球菌相关功能基因和调控通路在致病过程中的机制。
英文摘要:
      Objective:This study aims to investigate the in vitro effects of different oligotrophic conditions on the transcriptional level of Enterococcus faecalis(E.faecalis)during starvation phase under simulated root canal pressure. Methods:The E.faecalis(ATCC 29212)was induced to starvation phase and cultured under different oligotrophic conditions(TSB with 0%,0.05%,0.15%,and 0.25% glucose),gene transcription levels and metabolic pathways were measured after one week. Results:The results showed that there was no significant change in the gene transcription level in the 0.15% glucose group compared with the 0.25% group. The gene transcription level in the 0.05% glucose group and particular in the 0% glucose group changed significantly. In the 0% glucose group,GO function mostly involved in metabolic processes and catalytic activity,and there was no significant difference in KEGG pathway. The top three metabolic pathways were ABC transporter,PTS system and amino acid biosynthesis. Conclusion:The changes in gene transcription level of E.faecalis under oligotrophic conditions may be related to the survival and pathogenicity of E.faecalis under starvation conditions. We will further explore the mechanism of related functional genes and regulatory pathways of E.faecalis in the pathogenesis of the disease on the basis of this study.
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