文章摘要
蒋松辰,高尚远,谢发江,赵 佳,江 凤.ω⁃3多不饱和脂肪酸对心肌肥厚大鼠心肌细胞外基质重塑的影响[J].南京医科大学学报,2019,(7):960~965
ω⁃3多不饱和脂肪酸对心肌肥厚大鼠心肌细胞外基质重塑的影响
Effects of omega⁃3 polyunsaturated fatty acids on myocardial extracellular matrix remodeling in rats with cardiac hypertrophy
投稿时间:2018-11-21  
DOI:10.7655/NYDXBNS20190703
中文关键词: ω⁃3多不饱和脂肪酸  心肌肥厚  基质金属蛋白酶⁃9  基质金属蛋白酶抑制剂⁃1  纤连蛋白
英文关键词: n⁃3 PUFAs  cardiac hypertrophy  matrix metalloproteinase⁃9  matrix metalloproteinase inhibitor⁃1  fibronectin
基金项目:四川省教育厅科研课题(14ZA0137)
作者单位
蒋松辰 西南医科大学附属医院心血管内科四川 泸州 646000 
高尚远 西南医科大学附属医院心血管内科四川 泸州 646000 
谢发江 西南医科大学附属医院心血管内科四川 泸州 646000 
赵 佳 彭州市人民医院心血管内科四川 彭州 611930 
江 凤 西南医科大学附属医院心血管内科四川 泸州 646000 
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中文摘要:
      目的:探讨ω?3多不饱和脂肪酸(ω?3 polyunsaturated fatty acids,n?3PUFAs)对腹主动脉缩窄(abdominal aorta coarctation,AAC)术后心肌肥厚大鼠心肌细胞外基质重塑的影响。方法:雄性SD大鼠40只,随机分为假手术组、模型组。模型组大鼠行AAC术。术后1周,再随机将模型组分成AAC组、AAC+ n?3PUFAs低剂量组、AAC+ n?3PUFAs高剂量组,每组8只,后两组分别予以400 mg/(kg·d)及1 000 mg/(kg·d)的n?3PUFAs灌胃,假手术组和AAC组给予等体积生理盐水灌胃。8周后处死大鼠,留取心肌组织测定左心室质量指数(LVW/BW)和心脏质量指数(HW/BW),取心肌组织行HE染色及Masson染色,取血清用碱水解法测定羟脯氨酸含量,用免疫蛋白印迹(Western blot)法分析心肌组织基质金属蛋白酶9(matrix metalloproteinase 9,MMP?9)、基质金属蛋白酶抑制剂?1(tissue inhibitor of metalloproteinase?1,TIMP?1)及纤连蛋白(fibronectin,FN)的表达。结果:与假手术组相比,模型组LVW/BW、HW/BW、羟脯氨酸含量明显升高(P < 0.05),病理染色示心肌细胞肥大,胶原纤维增加,MMP?9表达增加,TIMP?1含量减少,FN表达增加,差异有统计学意义(P < 0.05)。与AAC组相比,AAC+ n?3PUFAs低剂量组、AAC+ n?3PUFAs高剂量组LVW/BW、HW/BW、羟脯氨酸含量降低,病理染色示心肌细胞肥大减轻、胶原含量减少,MMP?9、FN表达减少,TIMP?1表达增加(P < 0.05)。且AAC+ n?3PUFAs高剂量组较AAC+ n?3PUFAs低剂量组各项指标变化更为明显,差异均有统计学意义(P < 0.05)。结论:n?3PUFAs对心肌肥厚有保护作用,其机制可能与抑制MMP?9、FN的过度表达,上调TIMP?1的表达,影响心肌细胞外基质重塑相关。
英文摘要:
      Objective:To investigate the effects of omega?3 polyunsaturated fatty acids(n?3 PUFAs)on myocardial extracellular matrix remodeling in rats with myocardial hypertrophy after abdominal aortic coarctation(AAC). Methods:Forty male Sprague?Dawley rats were randomly divided into sham operation group(group A)and model group. AAC was performed in model group. One week after operation,the model group was randomly divided into three groups:AAC group(group B),AAC + n?3PUFAs low dose group(group C),AAC+ n?3PUFAs high dose group(group D). The group C and group D were given by gavage 400 mg/(kg·d)and 1 000 mg/(kg·d)n?3 PUFAs respectively,and the group A and group B were given an equal volume of normal saline. After 8 weeks,the rats were sacrificed,and the left ventricular mass index(LVW/BW)and cardiac mass index(HW/BW)were measured. The myocardial tissues were stained with HE and Masson. The serum hydroxyproline was determined by alkaline hydrolysis method. The content of matrix metalloproteinase?9(MMP?9),tissue inhibitor of metalloproteinase?1(TIMP?1)and fibronectin(FN)in myocardial tissue were analyzed by Western blot. Results:Compared with the group A,LVW/BW,HW/BW,and hydroxyproline content in groups B,C,and D increased significantly(P < 0.05). Pathological staining showed cardiomyocyte hypertrophy,collagen fiber and MMP?9 expression increasing. The expression of TIMP?1 decreased(P < 0.05). The expressions of FN in groups B,C and D were significantly higher than that in group A(P < 0.05). Compared with group B,LVW/BW,HW/BW,and hydroxyproline content in group C and group D decreased,the myocardial cells shown less hypertrophy by pathological staining and the collagen content also decreased,and MMP?9 and FN expression decreased,whereas TIMP?1 expression increased(P < 0.05). Compared with group C,the changes of all the above indexes in group D were greater(P < 0.05). All these differences have statistical significance. Conclusion:n?3PUFAs have protective effects on myocardial hypertrophy after abdominal aortic coarctation. The mechanism may be related to the inhibition of overexpression of MMP?9 and FN,the up?regulation of TIMP?1 expression,and the influence on the changes in extracellular matrix components.
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