文章摘要
詹晓芬,王少洪,王媛媛,吴 璇,肖坤胜.Van⁃clear环保透明脱蜡剂在不同宫颈病变组织HE染色、蛋白及基因表达检测中的应用[J].南京医科大学学报,2019,(7):1062~1066
Van⁃clear环保透明脱蜡剂在不同宫颈病变组织HE染色、蛋白及基因表达检测中的应用
Application of environmental transparent dewaxing agent Van⁃clear in HE staining,protein expression and gene amplification in different cervical lesions
投稿时间:2018-09-05  
DOI:10.7655/NYDXBNS20190726
中文关键词: Van⁃clear  二甲苯  免疫组织化学  P16蛋白  Ki67蛋白  hTERC  宫颈上皮内瘤变  宫颈癌
英文关键词: Van⁃clear  xylene  immunohistochemistry  P16 protein  Ki67 protein  hTERC gene  cervical intraepithelial neoplasia  cervical cancer
基金项目:汕头市科技计划医疗卫生类别项目(汕府科[2017]119号?18)
作者单位
詹晓芬 汕头市中心医院病理科广东 汕头 515031 
王少洪 汕头市中心医院病理科广东 汕头 515031 
王媛媛 汕头市中心医院病理科广东 汕头 515031 
吴 璇 汕头市中心医院病理科广东 汕头 515031 
肖坤胜 汕头市中心医院病理科广东 汕头 515031 
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中文摘要:
      目的:对比环保透明脱蜡剂Van?clear与传统透明脱蜡剂二甲苯在不同宫颈病变组织HE染色、P16及Ki67蛋白表达、hTERC基因表达检测效果的差异,探讨Van?clear的实用价值。方法:收集2016年1月至2018年7月于汕头市中心医院病理送检的宫颈炎标本42例、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)Ⅰ标本53例、CINⅡ标本61例、CINⅢ标本56例、宫颈癌标本48例,每例标本同一病变区域切取2块样本,随机分为A、B组。A组使用二甲苯制作石蜡切片260张;B组使用Van?clear制作石蜡切片260张。两组切片分别进行苏木素?伊红(hematoxylin eosin,HE)染色、P16和Ki67蛋白免疫组织化学(immunohistochemistry,IHC)染色、人端粒酶核糖核酸组分(human telomerase RNA component,hTERC)基因荧光原位杂交(fluorescence in situ hybridization,FISH)检测,分析两组切片HE染色优良率、P16及Ki67蛋白、hTERC基因表达阳性率的差异。结果:① A、B组切片HE染色、IHC染色均达到行业质控标准;②A、B组切片HE染色优良率分别为98.08%、99.23%,两组间优良率的差异无统计学意义(χ2=1.30,P=0.25);③IHC染色阳性率比较:A、B组切片相对应的不同宫颈病变组织中P16、Ki67蛋白阳性率差异均无统计学意义(均P>0.05);④hTERC基因阳性率比较:A、B组切片相对应的不同宫颈病变组织中hTERC基因表达阳性率差异无统计学意义(P>0.05),且两组间荧光显微镜下图像无明显差异。结论:Van?clear作为一种新型环保透明脱蜡剂,在不同宫颈病变组织HE染色、P16及Ki67蛋白表达、hTERC基因表达检测效果与传统透明脱蜡剂二甲苯相当,且绿色环保,建议在临床推广使用。
英文摘要:
      Objective:To compare the difference of Hematoxylin eosin(HE) staining,P16 and Ki67 protein expression,as well as hTERC gene hybridization effect between environmental transparent dewaxing agent Van?clear and traditional transparent dewaxing agent xylene in different cervical lesions,and to explore the practical value of Van?clear. Methods:A total of 42 cervicitis specimens,53 cervical intraepithelial neoplasia(CIN)Ⅰspecimens,61 CIN Ⅱ specimens,56 CIN Ⅲ specimens,and 48 cervical cancer specimens were collected from Shantou Central Hospital from January 2016 to July 2018. In group A,260 paraffin sections were made from xylene,while in group B,260 paraffin sections were made from Van?clear. HE staining,immunohistochemistry(IHC)staining,and fluorescence in situ hybridization(FISH)of hTERC gene were used to detect the difference of HE staining excellence rate,P16 and Ki67 protein expression,and hTERC gene positive rate between the two groups. Results:①Sections in group A and group B for HE staining and immunohistochemistry staining all met the quality control standard of industry. ②The excellent and good rates of HE staining in group A and group B were 98.08% and 99.23%,respectively. There was no significant difference between the two groups(χ2=1.30,P=0.25). ③Comparison of positive rate of IHC staining showed there was no significant difference in the positive rate of P16 and Ki67 protein in different cervical lesions between groups A and gronp B(P>0.05).④Comparison of the positive rate of hTERC gene amplification showed there was no significant difference between group A and gronp B in different cervical lesions(P>0.05),and no significant difference was observed between the two groups under fluorescence microscope. Conclusion:Van?clear,as a new environmental transparent dewaxing agent,has the same effect as traditional transparent dewaxing agent xylene in HE staining,P16 and Ki67 protein expression,and hTERC gene detection in different cervical lesions. It is also green and environment?friendly,we recommend that it be widely used in clinic.
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