Effects of experimental periodontitis on the expression of reactive oxygen species in marrow of mice
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摘要:
目的：研究实验性牙周炎对小鼠骨髓活性氧（reactive oxygen species ROS）生成的影响，初步探讨ROS在牙周炎中的作用。方法：选用8周龄C57BL/6雌性小鼠20只，采用丝线结扎法建立实验性牙周炎动物模型，在术后4周取材，对小鼠上颌骨进行micro-CT扫描、HE染色及TRAP染色；取小鼠四肢骨髓，通过流式细胞仪检测骨髓中ROS含量水平。结果：实验组术后4周，上颌第二磨牙从釉牙骨质界到牙槽嵴顶的距离增大，近远中及根分叉处牙槽骨吸收明显，成骨细胞减少；单位长度破骨细胞数显著增多（P < 0.001）；小鼠骨髓中ROS平均荧光强度显著高于对照组（P < 0.001）。结论：ROS含量在实验性牙周炎小鼠骨髓中增加，可能影响成骨和破骨细胞的分化，参与牙周炎骨吸收。
Abstract:
Objective：This study aims to investigate the effect of experimental periodontitis on the expression of reactive oxygen species（ROS） in marrow of mice. Methods：A total of 20 eight-week-old C57BL/6 mice were used in the study，and-experimental periodontitis was induced in mice by thread ligature. After 4 weeks of experiment，the maxilla were undergone micro-CT scanning，HE staining，tartrate-resistant acid phosphatase（TRAP）staining. Flow cytometry was employed to study the expression of ROS in marrow. Results：After four weeks of experimental periodontitis，alveolar bone resorption and TRAP positive osteoclast number were all increased. There were significantly increases of the ROS level in marrow compared with controls. Conclusion：Experimental periodontitis can increase the expression of ROS in mice marrow，which may play regulatory roles in alveolar bone loss.
