文章摘要
沈 阳,陈思遐,冯旰珠.栀子苷对RAW264.7细胞胞饮和噬菌功能双向调节作用的初步观察[J].南京医科大学学报,2019,(10):1426~1429
栀子苷对RAW264.7细胞胞饮和噬菌功能双向调节作用的初步观察
Preliminary observation of dual regulatory effects of geniposide on pinocytosis and phagocytosis of RAW246.7 cells
投稿时间:2019-06-14  
DOI:10.7655/NYDXBNS20191004
中文关键词: 栀子苷  RAW264.7细胞  胞饮活性  噬菌作用  铜绿假单胞菌
英文关键词: geniposide  RAW264.7 cells  pinocytosis  phagocytosis  Pseudomonas aeruginosa
基金项目:国家自然科学基金(81670013)
作者单位
沈 阳 南京医科大学第二临床医学院江苏 南京 210011 
陈思遐 南京医科大学第二临床医学院江苏 南京 210011 
冯旰珠 南京医科大学第二附属医院呼吸科江苏 南京 210011 
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中文摘要:
      目的:研究栀子苷对巨噬细胞系RAW264.7细胞活性和内吞功能的影响。方法:采用CCK?8法判定在不同浓度、不同时间下栀子苷对RAW264.7细胞增殖的影响;根据CCK?8检测结果,挑选适宜浓度栀子苷与RAW264.7细胞共孵育24 h后,检测RAW264.7细胞对中性红溶液的胞饮活性;进一步以2 mg/mL栀子苷与RAW264.7细胞共孵育后,检测RAW264.7细胞对铜绿假单胞菌的吞噬活性及胞内细菌存活情况。结果:栀子苷的细胞毒性小,在0~8 mg/mL浓度内对RAW264.7细胞的增殖无明显影响;2 mg/mL的栀子苷与细胞共孵育24 h后可明显促进静息状态下RAW264.7细胞的胞饮活性;2 mg/mL的栀子苷抑制RAW264.7细胞对铜绿假单胞菌的吞噬及胞内杀菌作用。结论:栀子苷对RAW264.7细胞的胞饮和噬菌功能具有双向调节作用。
英文摘要:
      Objective:To investigate the effect of geniposide on activity and endocytosis of murine macrophage RAW264.7 cells. Methods:The RAW264.7 cells were added with geniposide at different concentrations and further cultured for several hours. The proliferation of RAW264.7 cells was measured by CCK?8 assay. According to the results of CCK?8 assay,geniposide at appropriate concentrations were selected to culture with RAW264.7 cells for 24 h,and subsequently detected the pinocytosis activity of neutral red solution. After cultured with geniposide at the optimal concentration(2 mg/mL),RAW264.7 cells were detected the phagocytosis activity of Pseudomonas aeruginosa and intracellular bacterial survival. Results:Geniposide showed little cytotoxicity on RAW264.7 cells. The proliferation of RAW264.7 cells was not seriously affected by 0~8 mg/mL geniposide. Geniposide at 2 mg/mL could significantly promote the pinocytosis of neutral red solution by RAW264.7 cells,but inhibited the phagocytosis of Pseudomonas aeruginosa and intracellular bacterial survival. Conclusion:Geniposide has dual regulatory effect on the pinocytosis and phagocytosis of RAW264.7 cells.
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