文章摘要
张秀青,于春琳,刘 峰,赵德育.PDE4D在哮喘患儿和哮喘模型的表达差异[J].南京医科大学学报,2019,(11):1561~1566
PDE4D在哮喘患儿和哮喘模型的表达差异
Expression differences of PDE4D in asthmatic children and asthmatic models
投稿时间:2019-02-17  
DOI:10.7655/NYDXBNS20191102
中文关键词: PDE4D  哮喘  GEO  GWAS  肥大细胞
英文关键词: PDE4D  asthma  GEO  GWAS  mast cells
基金项目:江苏省青年医学人才项目(QNRC2016087);南京市科委(社发)(201723003);南京市卫计委重点项目(ZKX18041)
作者单位
张秀青 南京医科大学附属儿童医院呼吸科江苏 南京 210008 
于春琳 南京医科大学附属儿童医院呼吸科江苏 南京 210008 
刘 峰 南京医科大学附属儿童医院呼吸科江苏 南京 210008 
赵德育 南京医科大学附属儿童医院呼吸科江苏 南京 210008 
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中文摘要:
      目的:筛选哮喘相关候选基因,并在人群和动物水平验证。方法:从高通量基因表达数据库(gene expression omnibus,GEO)搜索哮喘痰相关基因芯片数据,利用GEO 2R筛选表达差异基因,并参考全基因关联分析(genome?wide association study,GWAS)筛选哮喘相关基因,收集哮喘患儿及同期健康儿童痰标本,构建小鼠哮喘模型、大鼠肥大细胞株(RBL?2H3)哮喘模型,应用RT?PCR进行验证。结果:共纳入2套哮喘痰基因芯片数据,选出有交集的差异表达基因190个,结合GWAS最终筛选出哮喘相关基因磷酸二酯酶4D(PDE4D)。进一步验证PDE4D在哮喘患儿痰样本中表达上调(P < 0.05),但在哮喘小鼠模型整个肺组织中表达下调(P < 0.05),而在致敏的RBL?2H3细胞中表达上调(P < 0.05)。结论:PDE4D在人群、哮喘小鼠及肥大细胞中存在差异表达,可能作为肥大细胞参与哮喘发展的研究靶点。
英文摘要:
      Objective:This study aims to screen for asthma?related candidate genes and validate at the population and animal levels to explore the pathogenesis of asthma. Methods:The asthma?related sputum chip was searched from Gene Expression Omnibus(GEO),the differentially expressed genes were screened by GEO 2R,and the asthma?related genes were screened by reference to the whole gene association analysis(GWAS). Asthmatic children and healthy children’s sputum specimens werecolle cted. Mouse asthma model construct and rat mast cell strain(RBL?2H3)asthma model were constructed,and real?time fluorescence quantitative polymerase chain reaction(RT?PCR)was used for differential expression verification. Results:Two sets of asthmatic gene chip data were included,and 190 differentially expressed genes with crossovers were selected. GWAS was used to finally screen out the asthma?related gene PDE4D. It was further verified that the expression of PDE4D was increased in the sputum samples of asthmatic children(P < 0.05),and decreased in the whole lung tissue of the asthma mouse model(P < 0.05),but up?regulated in the sensitized RBL?2H3 cells(P < 0.05). Conclusion:PDE4D is differentially expressed in human,asthmatic mice and mast cells,which may serve as a research target for mast cells to participate in the development of asthma.
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