文章摘要
汪 悦,顾建雨,朱奕名,张尧尧,孙 颖.内毒素耐受对小鼠腹腔巨噬细胞分泌MMP⁃1、MMP⁃7及细胞迁移能力的影响[J].南京医科大学学报,2020,(1):21~25
内毒素耐受对小鼠腹腔巨噬细胞分泌MMP⁃1、MMP⁃7及细胞迁移能力的影响
Effects of endotoxin tolerance on the secretion of MMP⁃1,MMP⁃7 in mice peritoneal macrophages and the migration of L929 cells
投稿时间:2019-06-14  
DOI:10.7655/NYDXBNS20200105
中文关键词: 牙龈卟啉单胞菌  内毒素耐受  巨噬细胞  基质金属蛋白酶⁃1  基质金属蛋白酶⁃7  细胞迁移
英文关键词: Porphyromonas gingivalis  endotoxin tolerance  macrophages  MMP⁃1  MMP⁃7  cell migration
基金项目:国家自然科学基金(81771075);江苏省自然科学基金(BK20161565);江苏省高校优势学科建设工程资助项目(2018?87)
作者单位
汪 悦 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
顾建雨 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
朱奕名 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
张尧尧 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
孙 颖 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙周科江苏 南京 210029 
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中文摘要:
      目的:观察牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)脂多糖(lipopolysaccharide,LPS)诱导的耐受对小鼠腹腔巨噬细胞分泌基质金属蛋白酶(matrix metalloproteinase,MMP)?1和MMP?7的影响,以及耐受巨噬细胞对小鼠成纤维细胞L929迁移能力的影响。方法:采用1 μg/mL P.gingivalis LPS重复刺激小鼠腹腔巨噬细胞,构建内毒素耐受模型,并以1 μg/mL大肠杆菌(Escherichia coli,E.coli)LPS作为阳性对照。收集细胞条件培养液上清,采用ELISA技术检测MMP?1和MMP?7表达水平。观察耐受巨噬细胞条件培养液对L929细胞划痕创伤构建后细胞迁移能力的影响。结果:P. gingivalis LPS重复刺激后,巨噬细胞MMP?1分泌水平较单次刺激组降低(P < 0.05),MMP?7分泌水平无明显变化(P > 0.05)。P. gingivalis LPS诱导的耐受巨噬细胞培养液上清刺激12、24 h后,L929细胞划痕修复面积大于单次刺激组培养液上清刺激后(P < 0.05)。结论:P. gingivalis LPS诱导的耐受能抑制小鼠腹腔巨噬细胞分泌MMP?1,促进L929细胞迁移。
英文摘要:
      Objective:To observe the effects of endotoxin tolerance induced by Porphyromonas gingivalis(P.gingivalis)on the secretion of metalloproteinase(MMP)?1 and MMP?7 in peritoneal macrophages of mice and the wound?healing abilities of L929 cells. Methods:Endotoxin tolerance in murine peritoneal macrophages was triggered by repeated 1 μg/mL P.gingivalis lipopolysaccharide(LPS)stimulations,and 1 μg/mL Escherichia coli(E.coli) LPS was served as positive control. Supernatants from tolerized macrophages were collected and levels of MMP?1 and MMP?7 were determined by ELISA. In addition,L929 cells were stimulated by supernatants from tolerized macrophages and their wound?healing abilities were evaluated by repair areas after scratch. Results:Secretion of MMP?1 in macrophages decreased significantly after repeated stimulation by P.gingivalis LPS compared with that treated by single P.gingivalis LPS stimulation(P < 0.05),while there were no differences in MMP?7 levels between the two groups(P > 0.05). Repaired areas after scratch(12 h,24 h)in L929 cells cultured with supernatants from P.gingivalis LPS?tolerized macrophages were bigger than those from P.gingivalis LPS non?tolerized macrophages(P < 0.05). Conclusion:Endotoxin tolerance triggered by P.gingivalis LPS contributes to the decreased secretion of MMP?1 in murine peritoneal macrophages and the enhanced migration of L929 cells.
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