文章摘要
夏 阳,陈慧敏,胡姝颖,孙剑飞,郭 宇,贾 璐.静磁场连续曝磁对牙髓干细胞增殖和分化的影响[J].南京医科大学学报,2020,(2):191~194
静磁场连续曝磁对牙髓干细胞增殖和分化的影响
Effects of continuous exposure to static magnetic field on the proliferation and differentiation of dental pulp stem cells
投稿时间:2019-03-17  
DOI:10.7655/NYDXBNS20200208
中文关键词: 静磁场  牙髓干细胞  增殖  分化
英文关键词: static magnetic field  dental pulp stem cells  proliferation  differentiation
基金项目:国家自然科学基金(81771044);中国博士后基金(2015M571647);江苏省博士后基金(1402044B);江苏高校优势学科建设工程资助项目(2018-87);江苏省青年医学人才(QNRC2016853);东南大学南京医科大学合作研究项目(2242018K3DN16)
作者单位
夏 阳 东南大学江苏省生物材料与器件重点实验室 江苏 南京 210009南京医科大学口腔疾病研究江苏省重点实验室 江苏 南京 210029 
陈慧敏 南京医科大学口腔疾病研究江苏省重点实验室 江苏 南京 210029 
胡姝颖 南京医科大学口腔疾病研究江苏省重点实验室 江苏 南京 210029 
孙剑飞 东南大学江苏省生物材料与器件重点实验室 江苏 南京 210009 
郭 宇 南京医科大学口腔疾病研究江苏省重点实验室 江苏 南京 210029 
贾 璐 南京医科大学口腔疾病研究江苏省重点实验室 江苏 南京 210029 
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中文摘要:
      目的:研究静磁场连续曝磁对牙髓干细胞增殖和分化的影响。方法:搭建静磁场下细胞培养装置,磁通强度(35±5)mT,以常规培养的细胞为对照组。用扫描电镜检测磁场下与常规培养细胞的形态,以CCK?8检测细胞的增殖情况,同时检测细胞的碱性磷酸酶活性,并进行茜素红染色以说明矿化物形成情况。结果:扫描电镜显示,磁性培养的细胞与常规培养的细胞在形态上没有显著差异;磁场下与常规培养下的细胞增殖情况也没有差异;但是磁场下细胞的碱性磷酸酶活性显著增高(P < 0.05),且矿化物的形成也显著增多(P < 0.05)。结论:静磁场连续曝磁对牙髓干细胞的形态和增殖没有影响,但促进了其成骨方向的分化。
英文摘要:
      Objective:This stady aims to observe the effects of continuous exposure to static magnetic field(SMF)on the proliferation and differentiation of dental pulp stem cells(DPSCs). Methods:The magnetic cell culture set was used with the flux intensity about (35 ± 5)mT. The normal cultured cells were used as control. The morphology of the cells was detected by scanning electron microscopy(SEM). The cell proliferation was detected by CCK?8,and the alkaline phosphatase activity(ALP)was also measured. Alizarin red staining(ARS) was used to detect the mineral synthesis. Results:There were no significant differences in SEM images and cell proliferation between magnetic cultured cells and normal cultured cells,but the ALP activity of magnetic cultured cells was significantly increased(P < 0.05),and the mineralization formation of cells under magnetic culture was significantly more than that of normal cultured cells(P < 0.05). Conclusion:Continuous exposure to SMF neither changed the cell morphology nor the cell proliferation,but significantly promoted the differentiation of DPSCs.
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