文章摘要
邓媛媛,王 军,姚佳希,余 静,陈旭锋,高 蓉.双酚S暴露对小鼠脑⁃肠⁃菌轴神经递质代谢稳态的影响[J].南京医科大学学报,2020,(5):663~668
双酚S暴露对小鼠脑⁃肠⁃菌轴神经递质代谢稳态的影响
Bisphenol S exposure affected the mice neurotransmitter metabolism homeostasis through the brain⁃gut⁃microbiota axis
投稿时间:2019-10-29  
DOI:10.7655/NYDXBNS20200509
中文关键词: 双酚S  脑⁃肠轴  肠道菌群  液质联用技术
英文关键词: bisphenol S  brain⁃gut axis  gut microbiota  UHPLC⁃MS/MS
基金项目:国家自然科学基金(81773484)
作者单位
邓媛媛 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
王 军 南京医科大学公共卫生学院毒理学系江苏 南京 211166 
姚佳希 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
余 静 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
陈旭锋 南京医科大学第一附属医院急诊医学中心江苏 南京 210029 
高 蓉 南京医科大学公共卫生学院卫生检验学系江苏 南京 211166 
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中文摘要:
      目的:探讨双酚S(bisphenol S,BPS)暴露对小鼠脑?肠?菌轴神经递质代谢稳态的影响。方法:通过皮下注射建立C57BL/6J雄性小鼠BPS亚慢性暴露模型,基于UHPLC?MS/MS液质联用技术检测前额皮层、血清和肠中色氨酸与酪氨酸通路神经递质的水平;通过16S rRNA基因测序分析技术检测小鼠肠道菌群的表达。结果:BPS暴露引起小鼠前额皮层、血清和肠道中色氨酸与酪氨酸通路多种神经递质代谢紊乱:色氨酸通路中,与对照组小鼠相比,BPS组小鼠前额皮层、血清及肠道中的5?羟色胺(5?hydroxytryptamine,5?HT)含量降低,3?羟基犬尿氨酸(3?HK)在皮层与血清中显著上升;酪氨酸通路中,BPS暴露组小鼠前额皮层中左旋多巴(L?dopa)、多巴胺(dopamine,DA)水平明显上升,而在肠道中显著降低。此外,BPS暴露小鼠肠道菌群出现区别于对照小鼠的差异性分布,且不动杆菌属、乳杆菌属和一种未分类的梭菌属在两组间比较具有显著性差异。结论:BPS暴露可通过脑?肠轴影响中枢及外周色氨酸通路与酪氨酸通路神经递质代谢,同时可造成肠道菌群紊乱。
英文摘要:
      Objective:To investigate the effect of bisphenol S(BPS)exposure on the homeostasis of neurotransmitter metabolism in the mice brain?gut?microbiota axis. Methods:A subchronic BPS exposure model using male C57BL/6J mice was established. The neurotransmitters in tryptophan and tyrosine pathways in the prefrontal cortex,serum and intestine were detected with UHPLC?MS/MS. The gut microbiota analysis was detected based on 16S rRNA gene sequencing. Results:BPS exposure resulted in neurotransmitter metabolism disorders of tryptophan and tyrosine pathways in the mice prefrontal cortex,serum and intestine. In the tryptophan pathway,when compared with the control group,5?HT was decreased in the prefrontal cortex(PFC),serum and intestine of the BPS?exposed group,while 3?HK was greatly increased in the PFC and serum. In the tyrosine pathway,BPS exposure significantly increased L?dopa and DA levels in the PFC,but decreased the counterparts in the intestine. Additionally,BPS exposure led to a differential distribution of gut microbiota in BPS?exposure mice. In the genus level,the Acinetobacter,Lactobacillus and an unclassified_Clostridiales exerted great differences between the two groups. Conclusion:BPS exposure can affect central and peripheral neurotransmitter metabolism in tryptophan pathway and tyrosine pathway through the brain?gut axis. Additionally,BPS exposure caused gut microbiota disturbance.
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