Roles of Mxi1⁃0 in apoptosis of SH⁃SY5Y cells induced by oxygen and glucose deprivation
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摘要:
目的：探讨Max作用蛋白1-0（max action protein 1-0，Mxi1-0）在氧糖剥夺（oxygen-glucose deprivation，OGD）诱导SH-SY5Y细胞凋亡中的作用。方法：利用Western blot检测SH-SY5Y细胞在OGD条件下Mxi1-0和线粒体自噬相关蛋白LC3-Ⅱ、p62、Tom20的表达水平。Mxi1-0 siRNA转染SH-SY5Y细胞，以CCK-8法和Annexin V/PI染色法检测Mxi1-0在OGD诱导细胞凋亡中的作用。结果：OGD条件下，SH-SY5Y细胞中Mxi1-0的表达先上升后下降。OGD处理使线粒体自噬相关蛋白LC3-Ⅱ表达升高，p62和Tom20蛋白表达下降。OGD条件下，Mxi1-0沉默可抑制OGD诱导的线粒体自噬，增强OGD诱导的SH-SY5Y细胞凋亡。结论：Mxi1-0通过增强线粒体自噬拮抗OGD诱导的SH-SY5Y细胞凋亡。
Abstract:
Objective：This study aims to investigate the role of max action protein 1-0（Mxi1-0） in SH-SY5Y cell apoptosis induced by oxygen-glucose deprivation（OGD）. Methods：The expression levels of Mxi1-0 and mitophagy related proteins LC3-Ⅱ，p62，Tom20 in SH-SY5Y cells under OGD conditions were detected by Western blot. Mxi1-0 siRNA was tranfected into SH-SY5Y cells. The expression of Mxi1-0 and mitophagy related proteins（LC3-Ⅱ，p62，Tom20） were detected in SH-SY5Y cells under OGD using Western blot. The role of Mxi1-0 in OGD-induced apoptosis was detected using CCK-8 method and Annexin V/PI staining. Results：The expression of Mxi1-0 increased firstly and then decreased in SH-SY5Y cells after OGD treatment. The expression of mitophagy related protein LC3-Ⅱ increasesed and the expression of p62 and Tom20 proteins decreased after OGD treatment. Under OGD conditions，Mxi1-0 silence reduced mitophagy in SH-SY5Y induced by OGD，and increase the apoptosis of SH-SY5Y induced by OGD. Conclusion：Mxi1-0 antagonizes OGD induced apoptosis of SH-SY5Y cells by enhancing mitochondrial autophagy.
