文章摘要
聂 萍,胡圳圳,袁 雅,吴伟玲,郑大同.Max作用蛋白1⁃0在氧糖剥夺诱导SH⁃SY5Y细胞凋亡中的作用[J].南京医科大学学报,2020,(6):816~820
Max作用蛋白1⁃0在氧糖剥夺诱导SH⁃SY5Y细胞凋亡中的作用
Roles of Mxi1⁃0 in apoptosis of SH⁃SY5Y cells induced by oxygen and glucose deprivation
投稿时间:2019-09-28  
DOI:10.7655/NYDXBNS20200608
中文关键词: Max作用蛋白1⁃0  氧糖剥夺  细胞凋亡  线粒体自噬  SH⁃SY5Y细胞
英文关键词: max interacting protein1⁃0  oxygen⁃glucose deprivation  apoptosis  mitophagy  SH⁃SY5Y cells
基金项目:江苏省妇幼健康科研项目(F201711)
作者单位
聂 萍 南京医科大学第二临床医学院儿科江苏 南京 210011 
胡圳圳 南京医科大学第二附属医院临床分子基因检测中心江苏 南京 210003 
袁 雅 南京医科大学第二临床医学院儿科江苏 南京 210011 
吴伟玲 南京医科大学第二临床医学院儿科江苏 南京 210011
南京医科大学第二附属医院儿童医学中心江苏 南京 210003 
郑大同 南京医科大学第二临床医学院儿科江苏 南京 210011
南京医科大学第二附属医院临床分子基因检测中心儿童医学中心江苏 南京 210003 
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中文摘要:
      目的:探讨Max作用蛋白1?0(max action protein 1?0,Mxi1?0)在氧糖剥夺(oxygen?glucose deprivation,OGD)诱导SH?SY5Y细胞凋亡中的作用。方法:利用Western blot检测SH?SY5Y细胞在OGD条件下Mxi1?0和线粒体自噬相关蛋白LC3?Ⅱ、p62、Tom20的表达水平。Mxi1?0 siRNA转染SH?SY5Y细胞,以CCK?8法和Annexin V/PI染色法检测Mxi1?0在OGD诱导细胞凋亡中的作用。结果:OGD条件下,SH?SY5Y细胞中Mxi1?0的表达先上升后下降。OGD处理使线粒体自噬相关蛋白LC3?Ⅱ表达升高,p62和Tom20蛋白表达下降。OGD条件下,Mxi1?0沉默可抑制OGD诱导的线粒体自噬,增强OGD诱导的SH?SY5Y细胞凋亡。结论:Mxi1?0通过增强线粒体自噬拮抗OGD诱导的SH?SY5Y细胞凋亡。
英文摘要:
      Objective:This study aims to investigate the role of max action protein 1?0(Mxi1?0) in SH?SY5Y cell apoptosis induced by oxygen?glucose deprivation(OGD). Methods:The expression levels of Mxi1?0 and mitophagy related proteins LC3?Ⅱ,p62,Tom20 in SH?SY5Y cells under OGD conditions were detected by Western blot. Mxi1?0 siRNA was tranfected into SH?SY5Y cells. The expression of Mxi1?0 and mitophagy related proteins(LC3?Ⅱ,p62,Tom20) were detected in SH?SY5Y cells under OGD using Western blot. The role of Mxi1?0 in OGD?induced apoptosis was detected using CCK?8 method and Annexin V/PI staining. Results:The expression of Mxi1?0 increased firstly and then decreased in SH?SY5Y cells after OGD treatment. The expression of mitophagy related protein LC3?Ⅱ increasesed and the expression of p62 and Tom20 proteins decreased after OGD treatment. Under OGD conditions,Mxi1?0 silence reduced mitophagy in SH?SY5Y induced by OGD,and increase the apoptosis of SH?SY5Y induced by OGD. Conclusion:Mxi1?0 antagonizes OGD induced apoptosis of SH?SY5Y cells by enhancing mitochondrial autophagy.
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