文章摘要
姜润浩,冷 斌,王 斌,邱 凯,黄新鑫,刘 圣,祖庆泉.犬脑组织灌流固定方法的改进[J].南京医科大学学报,2020,(6):821~825
犬脑组织灌流固定方法的改进
An improved perfusion technique for fixation of canine brain
投稿时间:2019-07-08  
DOI:10.7655/NYDXBNS20200609
中文关键词:   灌流固定  脑组织  介入放射
英文关键词: canines  fixation perfusion  brain  interventional radiology
基金项目:国家自然科学基金(81501565,81571777,81771794)
作者单位
姜润浩 南京医科大学第一附属医院介入放射科江苏 南京 210029 
冷 斌 南京医科大学第一附属医院介入放射科江苏 南京 210029 
王 斌 南京医科大学第一附属医院介入放射科江苏 南京 210029 
邱 凯 南京医科大学第一附属医院介入放射科江苏 南京 210029 
黄新鑫 南京医科大学第一附属医院介入放射科江苏 南京 210029 
刘 圣 南京医科大学第一附属医院介入放射科江苏 南京 210029 
祖庆泉 南京医科大学第一附属医院介入放射科江苏 南京 210029 
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中文摘要:
      目的:本研究对犬经心脏脑组织灌流方法进行改进,以提高固定效果。方法:7只比格犬随机分为3组:对照组1只,经心脏灌流法3只,改进灌流法3只。使用改进灌流法的实验犬在麻醉后行全身肝素化,穿刺股动脉后,在透视引导下经股动脉将导管插至一侧颈内动脉作为流入道,开放右心耳作为流出道,并结扎下腔静脉及腹主动脉,先灌注生理盐水,后灌注4%多聚甲醛。灌注结束后取出脑组织进行HE染色与电镜观察。结果:相比传统经心脏灌流法,改进灌流法的脑组织细胞自溶现象明显减少,且更加省液、省时。结论:改进后的灌流方法提高了犬脑组织固定效果,是一种可行有效的大动物脑组织灌流固定方法。
英文摘要:
      Objective:This study aims to modify the transcardial perfusion method to improve the effects of brain fixation in canines. Methods:Seven beagle dogs were randomly divided into three groups:control group(n=1),transcardial perfusion group(n=3),and improved perfusion group(n=3). Canines in the improved perfusion group were anaesthetized and heparin was administrated. The femoral artery access was achieved,then a catheter was inserted into internal carotid artery under fluoroscopic guidance and constituted the inflow,and the right auricle was opened to act as an outflow access. After the ligation of inferior vena cava and abdominal aorta,the brains were perfused with saline followed by paraformaldehyde. Once the perfusion was accomplished,the brains were harvested for HE staining and transmission electron microscopy. Results:Compared with the transcardial perfusion method,the improved perfusion method can significantly reduce the occurrence of cell autolysis,and it is liquid?saving and time?saving. Conclusion:The improved perfusion method is more effective than transcardial perfusion in canine brain tissue fixation,and it is feasible for brain fixation in large animals.
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