Objective：This work aimed to explore the feasibility of establishing ex vivo animal model of segmentectomy. Methods：The animal model of segmentectomy was established by ex vivo porcine lungs. Six different segments were separated by electrotome，and their intersegmental planes were completely removed in order to evaluate and analyze the degree of air leakage and modeling time between different segmentectomy. Results：We identified the normal anatomy of segments of pig lungs firstly，and all animal models of segmentectomy were successfully established，which could achieve a satisfying effect of the identification and separation of intersegmental plane. There was no significant difference in the level of air leakage among the six various segments（P > 0.05）. The modeling time of the a segment of right cranial lobe（RS1a）segment［（880±83）s］ was shorter than other segments［the b segment of right cranial lobe（RS1b）：（1 531±79）s，the a segment of left cranial lobe（LS1a）：（1 384±75）s，the b segment of left cranial lobe（LS1b）：（1 207±56）s，the levo-a segment of left caudal lobe（LS2La）：（1 273±94）s，the dextro-a segment of left caudal lobe（LS2Da）：（1 179±108）s］，and there were significant differences among them（P < 0.01），and RS1a also got a lower sample rejection rate（11.11%）compared to the average level（26.24%）. Conclusion：The RS1a in ex vivo porcine lung can be utilized as an ideal model of segmentectomy for experiments.