文章摘要
李广杰,陈永刚,张学良,耿 彬,安江东,董海涛,王栓科.硫酸钙/纳米羟基磷灰石免疫人工骨对兔结核性骨缺损治疗的研究[J].南京医科大学学报,2020,(8):1130~1134
硫酸钙/纳米羟基磷灰石免疫人工骨对兔结核性骨缺损治疗的研究
Therapeutic effect of calcium sulfate/nano hydroxyapatite⁃immunized artificial bone on tuberculous bone defects in rabbits
投稿时间:2019-09-16  
DOI:10.7655/NYDXBNS20200809
中文关键词: 硫酸钙  纳米羟基磷灰石  人工骨  骨结核  骨缺损
英文关键词: calcium sulfate  nanometer hydroxyapatite  artificial bone  bone tuberculosis  bone defect
基金项目:甘肃省民生科技计划项目(1303FCMA018);甘肃省基础研究创新群体项目(1308RJIA004);甘肃省自然科学基金项目(1010RJZA124)
作者单位
李广杰 兰州大学第一医院关节与运动创伤科甘肃 兰州 730000兰州大学第二临床医学院甘肃 兰州 730000 
陈永刚 兰州大学第二医院骨科甘肃 兰州 730000 
张学良 兰州大学第一医院关节与运动创伤科甘肃 兰州 730000 
耿 彬 兰州大学第二医院骨科甘肃 兰州 730000 
安江东 兰州大学第二医院骨科甘肃 兰州 730000 
董海涛 兰州大学第二医院骨科甘肃 兰州 730000 
王栓科 兰州大学第二医院骨科甘肃 兰州 730000 
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中文摘要:
      目的:通过制备硫酸钙/纳米羟基磷灰石免疫抗结核人工骨,治疗兔免疫骨结核,探讨纳米羟基磷灰石免疫抗结核骨实际应用的效果。方法:采用明胶和聚己内酯复合包裹法制备硫酸钙/纳米羟基磷灰石抗结核人工骨;通过注射结核因子建立兔右侧股骨免疫骨结核模型,对骨结核模型进行病灶清除术,在缺损部位植入硫酸钙/纳米羟基磷灰石抗结核免疫人工骨,并在术后7、14、28 d检测抗结核药血药浓度;术后第4、12周,光镜下观察骨修复情况。结果:成功制备硫酸钙/纳米羟基磷灰石抗结核骨,电子显微镜下免疫人工骨空隙基本均匀,抗结核药物分散在空隙之间。成功建立兔股骨结核模型,并形成骨结核空洞;术后28 d治疗组还维持一定的抗结核药血药浓度;术后4周结核骨治疗组患处软骨细胞增殖活跃,并出现骨性愈合;术后12周,治疗组缺损愈合完全,缺损已被抗结核人工骨诱导的骨组织修复;对照组骨损也出现愈合,但骨质愈合弱。抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色显示,术后12周,形成的破骨细胞的体积和数量减少,对照组的破骨数量多于治疗组。ELISA结果显示,治疗后血清C?反应蛋白(C?reactive protein,CRP)、白细胞介素(interleukin,IL)?8、IL?6、肿瘤坏死因子?α(tumor necrosis factor?α,TNF?α)水平均降低。结论:硫酸钙/纳米羟基磷灰石免疫抗结核骨能够有效地治疗兔免疫骨结核,并保持一段时间的血药浓度,成骨效果好。
英文摘要:
      Objective:To treat the rabbit model of bone tuberculosis through preparation of calcium sulphate/nano hydroxyapatite immune artificial bone,and discuss the actual effect of nanometer hydroxyapatite immune artificial bone application. Methods:Calcium sulfate/nanometer hydroxyapatite was prepared by gelatine and polyhexyl ester composite coating,the rabbit model of the right femoral immune bone tuberculosis was established by injecting tuberculosis factor. After the lesion in the bone tuberculosis model was removed,the calcium sulfate/nanometer hydroxyapatite artificial bone was plated into the defect site. The blood drug concentration of the treated group was detected at 7,14,28 days after surgery. At 4 weeks and 12 weeks after surgery,bone repair was observed under a microscope. Results:The anti?tuberculous bone of calcium sulfate/nanometer hydroxyapatite was successfully prepared,and the immune artificial bone space was basically uniform under the electron microscope,and the anti?tuberculosis drugs were dispersed between the spaces. The model of rabbit femoral tuberculosis was successfully established and the tuberculosis of bone tuberculosis was formed. In the treatment group,the blood drug concentration was maintained within 28 days after operation. Chondrocyte proliferation and the occurrence of bone healing of treatment group were active in 4 weeks after operation. At 12 weeks after surgery,the bone defect of treatment group was healed completely,and the defect was repaired by bone tissue induced by anti?tuberculous bone. The bone loss in the control group was also healed,but the bone healing was weak. Tartrate resistant acid phosphatase(TRAP)staining showed that the volume and number of osteoclasts formed decreased at 12 weeks after surgery,and the number of osteoclasts in the control group was greater than that in the treatment group. The ELISA results showed that serum C?reactive protein(CRP),interleukin?8(IL?8),interleukin?6(IL?6),tumor necrosis factor?α(TNF?α) levels decreased after treatment. Conclusion:The sulphate/nanometer hydroxyapatite immune anti?tuberculous bone can effectively treat rabbit immune bone tuberculosis,maintain the blood drug concentration for a period of time,and has a good osteogenic effect.
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