文章摘要
杨芷雯,顾嘉雯,袁礼婵,孟 丽,马俊青,陈文静.Trio对破骨细胞的调控作用[J].南京医科大学学报,2020,(9):1297~1301
Trio对破骨细胞的调控作用
Regulatory effects of Trio on osteoclasts
投稿时间:2020-02-23  
DOI:10.7655/NYDXBNS20200909
中文关键词: Trio  破骨细胞  细胞特异性敲除
英文关键词: Trio  osteoclast  cell⁃special knockout
基金项目:国家自然科学基金(81771029);江苏省高校优势学科建设工程(JX10531803)
作者单位
杨芷雯 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院口腔正畸科江苏 南京 210029 
顾嘉雯 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院口腔正畸科江苏 南京 210029 
袁礼婵 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院口腔正畸科江苏 南京 210029 
孟 丽 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院口腔正畸科江苏 南京 210029 
马俊青 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院口腔正畸科江苏 南京 210029 
陈文静 南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院口腔正畸科江苏 南京 210029 
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中文摘要:
      目的:构建破骨细胞特异性Trio基因敲除小鼠模型,探究Trio在骨改建过程中对破骨细胞的影响。方法:应用cre?loxp系统培育破骨细胞特异性Trio基因敲除小鼠,Micro?CT扫描小鼠股骨,股骨组织切片采用HE染色、抗酒石酸酸性磷酸酶染色观察成骨细胞及破骨细胞数量。使用核因子(nuclear factor,NF)?κB受体活化因子配体及巨噬细胞集落刺激因子诱导小鼠骨髓细胞分化为破骨细胞,观测Trio对破骨细胞形成的影响。结果:Trio基因敲除小鼠的体型小,Micro?CT显示骨密度及相关参数升高。组织学染色表明基因敲除小鼠股骨的骨量增加,破骨细胞变少。体外实验中,Trio基因敲除小鼠骨髓细胞诱导分化的破骨细胞数量减少。结论:成功构建破骨细胞特异性Trio基因敲除小鼠模型,Trio敲除后,小鼠骨量增加,破骨细胞的分化受到抑制。
英文摘要:
      Objective:This study aims to construct osteoclast?special Trio gene knockout mouse model and explore the effects of Trio on osteoclasts. Methods:Osteoclast?special Trio gene knockout mice were cultivated by cre?loxp recombination system. Micro?CT was used to scan femurs. The histologic sections of femurs were stained with HE staining,and tartrate resistant acid phosphatase staining to observe number of osteoblasts and osteoclasts. Moue bone marrow cells were treated with macrophage colony stimulating factor(M?CSF) and receptor activator of nuclear factor?κ B ligand(RANKL) for differentiation to discover the effects of Trio on osteoclasts. Results:The mice of Trio knockout group showed small body size. The result of Micro?CT revealed bone mass and related indexes increased. Histologic sections staining displayed that the bone mass of femur increased and osteoclasts decreased in gene knockout mice. In vitro,the number of osteoclasts differentiated from bone marrow cells decreased in mice of Trio knockout group. Conclusion:osteoclast?specific Trio gene knockout mouse model was successfully established. The bone mass of Trio gene knockout mice increased,and osteoclast differentiation was inhibited.
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