文章摘要
尚彦星,周 蒙,鄂裘恺,张晓倩,张学森.神经损伤诱导蛋白2在卵巢发育中的作用[J].南京医科大学学报,2021,(4):477~482
神经损伤诱导蛋白2在卵巢发育中的作用
Effects of nerve injury inducing protein 2 during ovarian development
投稿时间:2021-02-01  
DOI:10.7655/NYDXBNS20210401
中文关键词: CRISPR/Cas9  Ninj2  卵巢发育
英文关键词: CRISPR/Cas9  Ninj2  ovary development
基金项目:国家重点研发计划(2018YFC1004203)
作者单位
尚彦星 南京医科大学生殖医学国家重点实验室江苏 南京 211166 
周 蒙 南京医科大学生殖医学国家重点实验室江苏 南京 211166 
鄂裘恺 南京医科大学生殖医学国家重点实验室江苏 南京 211166 
张晓倩 南京医科大学生殖医学国家重点实验室江苏 南京 211166 
张学森 南京医科大学生殖医学国家重点实验室江苏 南京 211166 
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中文摘要:
      目的:通过基因敲除模型研究神经损伤诱导蛋白2(Ninjurin2,Ninj2)在小鼠卵巢发育过程的作用。方法:利用CRISPR/Cas9技术构建Ninj2基因敲除小鼠,通过HE染色、免疫荧光染色、体外受精(in vitro fertilization,IVF)以及脂多糖(lipopolysaccharide,LPS)暴露对敲除小鼠表型进行分析,研究Ninj2在小鼠卵巢发育中的作用。结果:成功构建Ninj2基因敲除小鼠模型;小鼠体重及相关生化指标检测结果显示,Ninj2纯合敲除鼠与野生型小鼠相比无显著差异;HE染色显示,Ninj2敲除小鼠在新生和成年卵巢中各级卵泡发育情况与野生型小鼠相比均无明显差别;IVF结果表明,Ninj2敲除小鼠早期胚胎发育正常;LPS对Ninj2敲除小鼠卵巢发育没有影响。结论:在正常生理或炎性条件下,敲除Ninj2不影响小鼠卵巢发育。
英文摘要:
      Objective:To construct nerve injury?inducing protein 2(Ninj2) knockout mice and study its role during mouse ovarian development. Methods:We generated Ninj2 knockout mice by CRISPR/Cas9 technology,and detected the role of Ninj2 in mouse ovarian development by HE staining,immunofluorescence,in vitro fertilization(IVF) and lipopolysaccharide(LPS) exposure. Results:Ninj2 knockout mouse model was successfully constructed. Body weight and detection of related biochemical indicators showed that there was no significant difference between Ninj2 homozygous knockout mice and wild?type mice;HE staining showed that there was no significant difference in follicular development between Ninj2 knockout mice and wild?type controls at all levels in newborn and adult ovaries;IVF results showed that the early embryos of Ninj2 knockout mice developed normally;LPS had no effect on ovarian development in Ninj2 knockout mice. Conclusion:Depletion of Ninj2 does not affect the mouse ovarian development in normal physiological or inflammatory condition.
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