Objective：To observe the expression of Nup107 in colon cancer tissues，and explore the relationship of Nup107 with the clinicopathological characteristics and prognosis of colon cancer patients. Methods：Ualcan database and real-time quantitative PCR were used to observe the expression level of Nup107 mRNA in colon cancer tissues. A total of 226 colon cancer patients from the Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University were selected. Immunohistochemical staining was used to detect the expression level of Nup107 protein in colon cancer tissues and normal colon tissues. The relationship of Nup107 expression level with the clinical characteristics and prognosis was analyzed based on clinical data. Preliminary study on the mechanism of Nup107 in colon cancer was performed by gene set enrichment analysis. Results：The mRNA and protein expression levels of Nup107 in colon cancer tissues were significantly higher than those in normal colonic mucosal tissues（P < 0.05）. The expression of Nup107 was significantly associated with tumor size（P=0.004），lymph node metastasis（P=0.002），distant metastasis（P=0.007），AJCC stage（P=0.003），and Ki67 expression（P=0.001）. Colon cancer patients with high level of Nup107 had poorer overall survival than those with low level of Nup107（P < 0.001）. Nup107 might be involved in basic transcription factors，mismatch repair，homologous recombination，ribosome，spliceosome，RNA degradation and other pathways in colon cancer. Conclusion：Nup107 is an independent risk factor for prognosis，and it is expected to provide new strategies for diagnosis and treatment of colon cancer.