2014—2016年无锡市HIV/AIDS病毒亚型及耐药变化分析
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江苏省研究生科研与实践创新计划(KYCX17_0184)


Analysis of HIV/AIDS virus subtypes and drug resistance in Wuxi from 2014 to 2016
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    摘要:

    目的:了解江苏省无锡市2014年和2016年调查随访的人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者/艾滋病患者(HIV/AIDS)人群病毒亚型及耐药的变化特征。方法:以2014年无锡市HIV/AIDS未接受抗病毒治疗人群为研究对象,开展HIV分子流行病学调查及随访研究。收集感染者的基本信息,并从其血液样本中提取DNA,通过巢式-PCR扩增HIV的pol、env和gag 3个基因片段并测序,综合判断感染者的HIV基因型。将pol序列提交至HIV耐药数据库进行耐药突变位点分析。2016年随访相同人群并筛选出接受抗病毒治疗人群,再次检测HIV亚型和耐药突变情况,分析两年间亚型及耐药变化情况。结果:共整理收集HIV/AIDS者117例,检测234例样本,检出CRF01_AE、CRF07_BC、B、CRF67_01B、CRF08_BC、CRF55_01B和CRF68_01B 7种HIV-1亚型和6种独特重组型。2014年原发性耐药率为4.95%,2016年获得性耐药率为5.75%。5例感染者耐药情况发生变化,其中1例亚型发生变异。结论:无锡市HIV/AIDS人群HIV-1亚型多样且复杂,亚型及耐药变化大,应继续加强对亚型及耐药突变监测。

    Abstract:

    Objective:To investigate the change of HIV subtypes and drug resistance in the follow-up HIV/AIDS population in Wuxi,Jiangsu in 2014 and 2016. Methods:HIV molecular epidemiological survey and follow-up study were carried out among HIV/AIDS patients who did not receive antiviral treatment in Wuxi in 2014. The basic information of the infected person was collected and DNA was extracted from blood samples. The pol,env,and gag gene fragments of HIV were amplified by nest-PCR and sequenced to determine the HIV genotype of the infected person. The pol sequence was submitted to the HIV drug resistance database for drug resistance mutation site analysis. In 2016,the same population was followed up and selected for antiviral treatment. HIV subtypes and drug resistance mutations were detected again,and the changes of subtypes and drug resistance in two years were analyzed. Results:A total of 117 HIV/AIDS cases were collected,and 234 samples were detected. Seven subtypes of HIV-1,CRF01_ AE,CRF07_ BC,B,CRF67_ 01B,CRF08_ BC,CRF55_ 01B,and CRF68_ 01B,and six unique recombinant types(URFs)were detected. The transmitted drug resistance rate was 4.95% in 2014,acquired drug resistance rate was 5.75% in 2016. The drug resistance of 5 cases changed,and one of the subtypes changed. Conclusion:The HIV-1 subtypes of HIV/AIDS patients in Wuxi are diverse and complex. The subtypes and drug resistance have changed greatly. Therefore,it is necessary to strengthen the surveillance of subtypes and drug resistance mutations.

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陈剑双,殷玥琪,成 浩,张 轩,袁德富,张晓轩,王晓敏,魏乾坤,王 蓓.2014—2016年无锡市HIV/AIDS病毒亚型及耐药变化分析[J].南京医科大学学报(自然科学版),2021,(4):612-616

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  • 收稿日期:2020-11-13
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  • 在线发布日期: 2021-04-29
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