文章摘要
陈剑双,殷玥琪,成 浩,张 轩,袁德富,张晓轩,王晓敏,魏乾坤,王 蓓.2014—2016年无锡市HIV/AIDS病毒亚型及耐药变化分析[J].南京医科大学学报,2021,(4):612~616
2014—2016年无锡市HIV/AIDS病毒亚型及耐药变化分析
Analysis of HIV/AIDS virus subtypes and drug resistance in Wuxi from 2014 to 2016
投稿时间:2020-11-13  
DOI:10.7655/NYDXBNS20210424
中文关键词: HIV感染者  艾滋病患者  病毒亚型  耐药突变
英文关键词: HIV infected person  AIDS patients  virus subtype  drug resistance variation
基金项目:江苏省研究生科研与实践创新计划(KYCX17_0184)
作者单位
陈剑双 东南大学公共卫生学院流行病与卫生统计学系江苏 南京 210009 
殷玥琪 东南大学公共卫生学院流行病与卫生统计学系江苏 南京 210009 
成 浩 无锡市新吴区疾病预防控制中心江苏 无锡 214028 
张 轩 无锡市疾病预防控制中心江苏 无锡 214000 
袁德富 东南大学公共卫生学院流行病与卫生统计学系江苏 南京 210009 
张晓轩 东南大学公共卫生学院流行病与卫生统计学系江苏 南京 210009 
王晓敏 东南大学公共卫生学院流行病与卫生统计学系江苏 南京 210009 
魏乾坤 东南大学公共卫生学院流行病与卫生统计学系江苏 南京 210009 
王 蓓 东南大学公共卫生学院流行病与卫生统计学系江苏 南京 210009 
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中文摘要:
      目的:了解江苏省无锡市2014年和2016年调查随访的人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者/艾滋病患者(HIV/AIDS)人群病毒亚型及耐药的变化特征。方法:以2014年无锡市HIV/AIDS未接受抗病毒治疗人群为研究对象,开展HIV分子流行病学调查及随访研究。收集感染者的基本信息,并从其血液样本中提取DNA,通过巢式?PCR扩增HIV的pol、env和gag 3个基因片段并测序,综合判断感染者的HIV基因型。将pol序列提交至HIV耐药数据库进行耐药突变位点分析。2016年随访相同人群并筛选出接受抗病毒治疗人群,再次检测HIV亚型和耐药突变情况,分析两年间亚型及耐药变化情况。结果:共整理收集HIV/AIDS者117例,检测234例样本,检出CRF01_AE、CRF07_BC、B、CRF67_01B、CRF08_BC、CRF55_01B和CRF68_01B 7种HIV?1亚型和6种独特重组型。2014年原发性耐药率为4.95%,2016年获得性耐药率为5.75%。5例感染者耐药情况发生变化,其中1例亚型发生变异。结论:无锡市HIV/AIDS人群HIV?1亚型多样且复杂,亚型及耐药变化大,应继续加强对亚型及耐药突变监测。
英文摘要:
      Objective:To investigate the change of HIV subtypes and drug resistance in the follow?up HIV/AIDS population in Wuxi,Jiangsu in 2014 and 2016. Methods:HIV molecular epidemiological survey and follow?up study were carried out among HIV/AIDS patients who did not receive antiviral treatment in Wuxi in 2014. The basic information of the infected person was collected and DNA was extracted from blood samples. The pol,env,and gag gene fragments of HIV were amplified by nest?PCR and sequenced to determine the HIV genotype of the infected person. The pol sequence was submitted to the HIV drug resistance database for drug resistance mutation site analysis. In 2016,the same population was followed up and selected for antiviral treatment. HIV subtypes and drug resistance mutations were detected again,and the changes of subtypes and drug resistance in two years were analyzed. Results:A total of 117 HIV/AIDS cases were collected,and 234 samples were detected. Seven subtypes of HIV?1,CRF01_ AE,CRF07_ BC,B,CRF67_ 01B,CRF08_ BC,CRF55_ 01B,and CRF68_ 01B,and six unique recombinant types(URFs)were detected. The transmitted drug resistance rate was 4.95% in 2014,acquired drug resistance rate was 5.75% in 2016. The drug resistance of 5 cases changed,and one of the subtypes changed. Conclusion:The HIV?1 subtypes of HIV/AIDS patients in Wuxi are diverse and complex. The subtypes and drug resistance have changed greatly. Therefore,it is necessary to strengthen the surveillance of subtypes and drug resistance mutations.
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