Objective：To investigate the glucose metabolism characteristics of CD4+CD25+CD127low regulatory T cells（Treg）and CD4+CD25-CD127+ effector T cells（Teff）in growth environment of ovarian cancer cells. Methods：Fluorescence-activated cell sorting（FACS）separated CD4+ Treg cells and CD4+ Teff cells，then established a coculture system of human T cells with ovarian cancer cell SKOV3. The expression levels of glucose metabolism related genes and proteins were determined by real-time fluorescence quantitative PCR（RT-PCR）and Western blot. The levels of glucose uptake and glycolysis were detected by colorimetry. Results：Compared with the CD4+ Treg cells in single culture without SKOV3 and CD4+ Teff cells，the expression levels of glucose metabolism related genes and proteins in CD4+ Treg cells cocultured with SKOV3 were elevated（P＜0.05）and the levels of glucose uptake and glycolysis were increased［（59.78±2.57）pmol vs.（44.93±1.86）pmol，P＜0.05；（15.31 ± 2.05）mmol/L vs.（7.98 ±0.88）mmol/L，P＜0.05］. Conclusion：The level of glucose metabolism in CD4+ Treg cells is higher than CD4+ Teff cells in ovarian cancer cell growth environment，ovarian cancer cells could promote the expression levels of glucose metabolism related genes and proteins in CD4+ Treg cells.