Preliminary study on the inhibitory effects of active ingredient lawsone in Chinese medicine on triple negative breast cancer
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摘要:
目的：探讨中草药散沫花中活性成分散沫花素（lawsone）在三阴性乳腺癌（triple negative breast cancer，TNBC）治疗中的应用前景。方法：选取2株TNBC细胞系MDA-MB-231和SUM1315作为研究对象，通过CCK-8实验、划痕实验、Transwell实验、平板克隆、EdU以及细胞周期检测等实验探讨散沫花素对乳腺癌细胞的作用。结果：MDA-MB-231和SUM1315的72 h半数抑制浓度（IC50）分别为249.87 μmol/L和158.34 μmol/L，散沫花素能明显抑制乳腺癌细胞迁移、侵袭及增殖，同时能延长乳腺癌细胞周期的G1期。结论：散沫花素可能通过抑制乳腺癌细胞迁移、侵袭及增殖来影响乳腺癌的发生发展。
Abstract:
Objective：This stndy aims to investigate the application prospect of lawsone in the treatment of triple negative breast cancer（TNBC）. Methods：Two TNBC cell lines，MDA-MB-231 and SUM1315，were selected as the research subjects. The effects of lawsone on breast cancer cells were investigated by CCK-8 assay，wound-healing assay，cellular invasion assay，colony formation assay，EdU assay and cell cycle assay. Results：The 72-hour IC50 values of MDA-MB-231 and SUM1315 were 249.87 μmol/L and 158.34 μmol/L，respectively. Lawsone could significantly inhibit the migration，invasion and proliferation of breast cancer cells，and prolong the G1 phase of breast cancer cell cycle. Conclusion：Lawsone may affect the occurrence and development of breast cancer by inhibiting the migration，invasion and proliferation of breast cancer cells.
