Objective：To investigate the impact of ABVD（adriamycin，bleomycin，vinblastine，dacarbazine） chemotherapy regime on the testicular 18F-fluorodeoxyglucose（FDG） uptake in patients with Hodgkin’s lymphoma（HL）. Methods：Thirty-four patients at diagnosis monitored with 18F-FDG on positron emission tomography（PET）/computed tomography（CT） to assess treatment response for HL were selected for this retrospective analysis. The patients received ABVD chemotherapy regimen for six courses. The four 18F-FDG PET/CT examinations were performed at baseline and after every two courses of chemotherapy. Blood glucose，body weight，injection dose and injection time were collected，and the maximum standardized uptake value（SUVmax） of testis and mediastinal blood pool were measured. The changes of testicular 18F-FDG uptake in patients with HL after four 18F-FDG PET/CT examinations were analyzed. Results：There were statistical significance in testis SUVmax and weight（P=0.017，0.001） at four PET/CT examinations for 34 cases with HL. Compared with the testicular SUVmax at the first PET/CT examination，the testicular SUVmax at the second time decreased by 0.998，P=0.045. There were no significant differences in testicular SUVmax between the last two time points and the first time or over the course of chemotherapy（P＞0.05）. The testis SUVmax were found to be positively correlated with mediastinal blood pool SUVmax at the first PET/CT examination（baseline）（r=0.51，P=0.002），but not with age（P=0.646）. The weight at last three PET/CT was higher than at the first time（P=0.009，0.004，0.016），but no changes were found during at last three PET/CT examinations（P > 0.05）. Conclusion：For patients undergoing ABVD chemotherapy regime for Hodgkin’s lymphoma，the testicular 18F-FDG uptake decreased temporarily over the course of chemotherapy，but recovered in the later. Therefore，ABVD chemotherapy regime may have little effect on testicular glycometabolism of patients with Hodgkin’s lymphoma.