文章摘要
楚瑞琦,曹峻岭,张军芳,谢龙,岳燕,胡小平,李思远,谭升顺.雪腐镰刀菌烯醇对培养软骨细胞代谢的影响及硒的保护作用[J].南京医科大学学报,2003,17(3):
雪腐镰刀菌烯醇对培养软骨细胞代谢的影响及硒的保护作用
The Effect of Nivalenol on Metabolism of Cultured Chondrocytes and the Protection of Selenium
  
DOI:10.7655
中文关键词: 雪腐镰刀菌烯醇、软骨细胞、硒
英文关键词: nivalenol  chondrocyte  selenium
基金项目:
楚瑞琦  曹峻岭  张军芳  谢龙  岳燕  胡小平  李思远  谭升顺
西安交通大学医学院第二医院皮肤科,西安,710004,中国;西安交通大学医学院第二医院皮肤科,西安,710004,中国;西安交通大学医学院第二医院皮肤科,西安,710004,中国;西安交通大学医学院第二医院皮肤科,西安,710004,中国;西安交通大学医学院第二医院皮肤科,西安,710004,中国;西安交通大学医学院第二医院皮肤科,西安,710004,中国;西安交通大学医学院第二医院皮肤科,西安,710004,中国;西安交通大学医学院第二医院皮肤科,西安,710004,中国;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, P. R. China;
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中文摘要:
      目的:探索雪腐镰刀菌烯醇(NIV)对培养软骨细胞代谢的影响及硒的保护作用.方法:应用生物化学方法检测NIV对培养的单层软骨细胞代谢的影响,软骨细胞单层培养、应用琼脂糖电泳和碱性单细胞微量凝胶电泳检测培养的单层软骨细胞DNA的损伤.结果:在实验设定的NIV浓度范围内(0.000 5~0.020 0 mg/L),随NIV浓度增加,软骨细胞基质蛋白多糖代谢明显降低、蛋白质合成减少、DNA含量减少、DNA受损细胞增多、DNA损伤加重,同浓度NIV加硒组损伤情况较未加硒组轻.在设定3个NIV浓度下,NIV并不影响软骨细胞的脂质过氧化,但加硒可使脂质过氧化物含量明显减低.结论:在实验设定的NIV浓度范围内,NIV对培养软骨细胞有明显损伤作用,加硒有保护作用,但不能阻止损伤.
英文摘要:
      Objective: To investigate the possible effect of nivalenol on metabolism of the cultured chondrocytes and the protection of selenium. Methods:The quantitative analyses of metabolism in single-layer cultured chondrocytes were performed by biochemical means and the impairment of DNA was observed by both of the single cell microgel electrophoresis assay and the agarose gel electrophoresis assay. Results: In the media containing different concentrations of nivalenol (0. 000 5-0. 020 0 mg/L), the amounts of DNA and proteoglycan in matrix of the chondrocytes were decreased. The synthesis of protein was reduced and the impairment of DNA deteriorated with the increase of the concentrations of nivalenol in the given dose. When selenium was added into the media, the impairment by nivalenol was decreased. In the media containing different concentrations of nivalenol, however, the lipid peroxidation of the chondrocytes was not affected by nivalenol, yet the amount of lipid peroxides significantly declined. Conclusion: Nivalenol may evidently cause impairment of the chondrocytes when its concentrations are in the present experimental range. Selenium can protect cultured chondrocytes, but cannot prevent their DNA from being impaired.
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