| Rong Zhou,Lin Lin,Yingchun Li,Yingbin Ge.[J].南京医科大学学报,2005,19(2): |
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| Expression of nitric oxide synthase in the colon of diabetic rats |
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| DOI：10.7655 |
| 中文关键词: |
| 英文关键词: diabetes nitric oxide synthases colonic dysfunction immunohistochemisty RT-PCR rat |
| 基金项目: |
| Rong Zhou Lin Lin Yingchun Li Yingbin Ge |
| Department of Physiology, Nanjing Medical University, Nanjing 210029, China;Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;Department of Physiology, Nanjing Medical University, Nanjing 210029, China;Department of Physiology, Nanjing Medical University, Nanjing 210029, China |
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| 中文摘要: |
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| 英文摘要: |
| Objective: To investigate the different expression of three isozymes of nitric oxide synthase (NOS) in diabetic rat colons and the contribution to the colonic dysfunction. Methods: Sprague-Dawley (SD) rats were used in this experiment and diabetes were induced by streptozotocin (65 mg/kg, i.v. ). Three isozymes of NOS (nNOS, iNOS and eNOS) expression in proximal and distal colon were measured in two weeks after diabetes induction using the methods of immunocytochemistry and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: Positive immunoreactivity for nNOS was found in intermuscular and submucous plexus neuronal cells, neither eNOS nor iNOS had been found in any layers of colon in the two groups. The expression of nNOS mRNA was significantly increased in diabetic colon than that in control rats as determined by RT-PCR. The eNOS mRNA level of diabetic colon was lower compared to thecontrol rats, while no expression of iNOS mRNA was found in the normal or diabetic rats. Conclusion: This report has demonstrated that nNOS increased and eNOS decreased in rat colon in the early stages of diabetes. NO production by the nNOS might play a key role in colonic dysfunction, as supported by raised nNOS mRNA and enzyme expression in the diabetic colon. Reduced eNOS activity might also contribute to colonic dysfunction in experimental diabetes. |
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