文章摘要
Qixing Gong,Qinhe Fan,Zhihong Zhang,Weiming Zhang.[J].南京医科大学学报,2005,19(3):
Detection of EWS-FLI1 fusion transcripts in paraffin embedded tissues of peripheral primitive neuroectodermal tumors by nested reverse transcription polymerase chain reaction
  
DOI:10.7655
中文关键词: 
英文关键词: peripheral primitive neuroectodermal tumor  EWS-FLI1 fusion gene  nested RT-PCR
基金项目:
Qixing Gong  Qinhe Fan  Zhihong Zhang  Weiming Zhang
Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
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中文摘要:
      
英文摘要:
      Objective: To assess the feasibility and significance of detecting EWS-FLIlfusion transcripts in paraffin embedded tissues of peripheral primitive neuroectodermal tumors (PNETs) by nested reverse transcription polymerase chain reaction (RT-PCR).Methods: Twelve formalin-fixed and paraffin-embedded (FFPE) samples of PNET were retrieved from archive and consultation materials,together with eight cases of controlled tumor. EWS-FLI1 fusion transcripts were detected by nested RT-PCR. Home-keeping gene β-actin was used to detect the quality of mRNA. Results: β-actin mRNA was detected in 9 of the 12 tumor cases. EWS-FLI1 fusion transcripts were detected in 6 cases, among which 4 had a "type 1" fusion transcript and 2 had a "type 2" fusion transcript. None of the controlled tumor was detected the fusion gene. Conclusion: RT-PCR is a feasible method for the detection of EWS-FLI1 fusion transcripts in FFPE tissues in PNET and the result is meaningful in differential diagnosis and prognostic evaluation.
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